Garabedian M J, Hung M C, Wensink P C
Proc Natl Acad Sci U S A. 1985 Mar;82(5):1396-400. doi: 10.1073/pnas.82.5.1396.
The adjacent and divergently transcribed yp1 and yp2 genes of Drosophila were separated at a site that is 342 base pairs upstream of yp2 and 883 base pairs upstream of yp1. Each gene was separately used to transform Drosophila germ-line-producing flies with a single copy of the introduced gene. Transcripts from the introduced genes were found only in adult females. Thus, the introduced genes maintain the sex- and time-specific expression pattern of the endogenous yp genes. However, the pattern of tissue-specific expression differed among the two introduced genes and the endogenous genes. Transcripts from both of the endogenous genes are found in fat bodies and ovaries. In contrast, transcripts from the introduced yp1 gene are found only in fat bodies, and those from the introduced yp2 gene are found only in ovaries. Thus, each introduced DNA segment lacks at least one of the cis-acting elements required for the normal pattern of tissue-specific expression. These results indicate that the expression of a yp gene in different tissues is determined by different cis-acting elements.
果蝇相邻且转录方向相反的yp1和yp2基因在一个位点处被分开，该位点在yp2上游342个碱基对处，在yp1上游883个碱基对处。每个基因分别用于转化果蝇生殖系产生的果蝇，引入的基因以单拷贝形式存在。引入基因的转录本仅在成年雌性中发现。因此，引入的基因维持了内源性yp基因的性别和时间特异性表达模式。然而，两个引入基因和内源性基因之间的组织特异性表达模式有所不同。两个内源性基因的转录本在脂肪体和卵巢中均有发现。相比之下，引入的yp1基因的转录本仅在脂肪体中发现，引入的yp2基因的转录本仅在卵巢中发现。因此，每个引入的DNA片段都缺乏正常组织特异性表达模式所需的至少一种顺式作用元件。这些结果表明，yp基因在不同组织中的表达由不同的顺式作用元件决定。
