显微注射的免疫球蛋白基因在转基因小鼠脾脏中的表达。
Expression of a microinjected immunoglobulin gene in the spleen of transgenic mice.
作者信息
Brinster R L, Ritchie K A, Hammer R E, O'Brien R L, Arp B, Storb U
出版信息
Nature. 1983;306(5941):332-6. doi: 10.1038/306332a0.
Abstract
Transgenic mice were produced by microinjection of a rearranged, functional immunoglobulin kappa gene into fertilized mouse eggs and implantation of the microinjected embryos into foster mothers. Mice that integrated the injected gene were mated and the DNA, RNA and serum kappa chains of their offspring were analysed. The data from offspring of three different transgenic mice indicate that the microinjected gene is expressed in the spleen, but not the liver of mice which inherited the injected gene.
摘要
通过将重排的功能性免疫球蛋白κ基因显微注射到受精的小鼠卵中，并将显微注射后的胚胎植入代孕母鼠体内，培育出了转基因小鼠。整合了注射基因的小鼠进行交配，并对其后代的DNA、RNA和血清κ链进行了分析。来自三只不同转基因小鼠后代的数据表明，显微注射的基因在继承了注射基因的小鼠的脾脏中表达，但在肝脏中不表达。
相似文献
1
Expression of a microinjected immunoglobulin gene in the spleen of transgenic mice.显微注射的免疫球蛋白基因在转基因小鼠脾脏中的表达。
Nature. 1983;306(5941):332-6. doi: 10.1038/306332a0.
2
High expression of cloned immunoglobulin kappa gene in transgenic mice is restricted to B lymphocytes.克隆免疫球蛋白κ基因在转基因小鼠中的高表达仅限于B淋巴细胞。
Nature. 1984;310(5974):238-41. doi: 10.1038/310238a0.
3
Rearrangement and expression of rabbit immunoglobulin kappa light chain gene in transgenic mice.兔免疫球蛋白κ轻链基因在转基因小鼠中的重排与表达。
Proc Natl Acad Sci U S A. 1987 Jun;84(12):4229-33. doi: 10.1073/pnas.84.12.4229.
4
Allelic exclusion and control of endogenous immunoglobulin gene rearrangement in kappa transgenic mice.κ转基因小鼠中内源性免疫球蛋白基因重排的等位基因排斥与调控
Nature. 1984;312(5994):517-20. doi: 10.1038/312517a0.
5
Transmission and expression of a specific pair of rearranged immunoglobulin mu and kappa genes in a transgenic mouse line.特定重排免疫球蛋白μ和κ基因在转基因小鼠品系中的传递与表达。
Nature. 1985;314(6009):330-4. doi: 10.1038/314330a0.
6
Specific expression of transferred genes. Foreign genes, which were transferred into mice, appear to be expressed according to more normal patterns of tissue distribution.转入基因的特异性表达。转入小鼠体内的外源基因似乎按照更正常的组织分布模式进行表达。
Science. 1983 Dec 2;222(4627):1001-2. doi: 10.1126/science.6417788.
7
Pre-B cells in kappa-transgenic mice.κ转基因小鼠中的前B细胞。
Nature. 1985;316(6026):356-8. doi: 10.1038/316356a0.
8
RNA splicing generates a variant light chain from an aberrantly rearranged kappa gene.RNA剪接从异常重排的κ基因产生一种可变轻链。
Nature. 1980 Aug 21;286(5775):776-9. doi: 10.1038/286776a0.
9
Two regulatory elements for immunoglobulin kappa light chain gene expression.免疫球蛋白κ轻链基因表达的两个调控元件。
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7041-5. doi: 10.1073/pnas.81.22.7041.
10
Organization and expression of immunoglobulin genes in fetal liver hybridomas.胎儿肝脏杂交瘤中免疫球蛋白基因的组织与表达
Proc Natl Acad Sci U S A. 1981 Jan;78(1):247-51. doi: 10.1073/pnas.78.1.247.
引用本文的文献
1
Concatenation of Transgenic DNA: Random or Orchestrated?转基因 DNA 的串联：随机还是有序？
Genes (Basel). 2021 Dec 10;12(12):1969. doi: 10.3390/genes12121969.
2
B Cells Carrying Antigen Receptors Against Microbes as Tools for Vaccine Discovery and Design.针对微生物的抗原受体携带 B 细胞作为疫苗发现和设计的工具。
Curr Top Microbiol Immunol. 2020;428:165-180. doi: 10.1007/82_2019_156.
3
Human monoclonal antibodies from transgenic mice.来自转基因小鼠的人源单克隆抗体。
Handb Exp Pharmacol. 2008;181(181):69-97. doi: 10.1007/978-3-540-73259-4_4.
4
In vitro comparison of the antigen-binding and stability properties of the various molecular forms of IgA antibodies assembled and produced in CHO cells.在体外对CHO细胞中组装并产生的各种分子形式的IgA抗体的抗原结合特性和稳定性进行比较。
Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):3029-34. doi: 10.1073/pnas.96.6.3029.
5
Increasing the chemical potential of the germ-line antibody repertoire.增加种系抗体库的化学势。
Proc Natl Acad Sci U S A. 1993 May 1;90(9):4008-11. doi: 10.1073/pnas.90.9.4008.
6
Gene transfer efficiency during gestation and the influence of co-transfer of non-manipulated embryos on production of transgenic mice.妊娠期的基因转移效率以及未处理胚胎共转移对转基因小鼠生产的影响。
Transgenic Res. 1994 Jan;3(1):20-5. doi: 10.1007/BF01976023.
7
Transgenic mice expressing a chimaeric anti-E. coli immunoglobulin alpha heavy chain gene.表达嵌合抗大肠杆菌免疫球蛋白α重链基因的转基因小鼠。
Transgenic Res. 1994 May;3(3):167-75. doi: 10.1007/BF01973984.
8
Introduction of genes into the germ line of animals.将基因导入动物种系。
Harvey Lect. 1984;80:1-38.
9
Developmental fate of a human insulin gene in a transgenic mouse.人类胰岛素基因在转基因小鼠中的发育命运
Mol Gen Genet. 1984;198(2):128-38. doi: 10.1007/BF00328712.
10
Transgenic mice harboring SV40 T-antigen genes develop characteristic brain tumors.携带SV40 T抗原基因的转基因小鼠会患上特征性脑肿瘤。
Cell. 1984 Jun;37(2):367-79. doi: 10.1016/0092-8674(84)90367-2.
本文引用的文献
1
Mouse oocytes transcribe injected Xenopus 5S RNA gene.小鼠卵母细胞转录注射的非洲爪蟾5S RNA基因。
Science. 1981 Jan 23;211(4480):396-8. doi: 10.1126/science.7194505.
2
Differential regulation of metallothionein-thymidine kinase fusion genes in transgenic mice and their offspring.金属硫蛋白-胸苷激酶融合基因在转基因小鼠及其后代中的差异调控。
Cell. 1982 Jun;29(2):701-10. doi: 10.1016/0092-8674(82)90186-6.
3
Immunoglobulin messenger RNAs of T lymphocytes.T淋巴细胞的免疫球蛋白信使核糖核酸
Mol Immunol. 1980 Jul;17(7):947-57. doi: 10.1016/0161-5890(80)90043-7.
4
Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes.由显微注射金属硫蛋白-生长激素融合基因的卵发育而成的小鼠显著生长。
Nature. 1982 Dec 16;300(5893):611-5. doi: 10.1038/300611a0.
5
Introduction of a rabbit beta-globin gene into the mouse germ line.将兔β-珠蛋白基因导入小鼠种系。
Nature. 1981 Nov 5;294(5836):92-4. doi: 10.1038/294092a0.
6
Enhancer elements in immunoglobulin genes.免疫球蛋白基因中的增强子元件。
Nature. 1983 May 26;303(5915):281-2. doi: 10.1038/303281a0.
7
Chromatin changes accompany immunoglobulin kappa gene activation: a potential control region within the gene.染色质变化伴随免疫球蛋白κ基因激活：该基因内的一个潜在控制区域。
Nature. 1982 Sep 30;299(5882):449-51. doi: 10.1038/299449a0.
8
Microinjection of a rabbit beta-globin gene into zygotes and its subsequent expression in adult mice and their offspring.将兔β-珠蛋白基因显微注射到受精卵中及其在成年小鼠及其后代中的后续表达。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6376-80. doi: 10.1073/pnas.78.10.6376.
9
Mapping of immunoglobulin variable region genes: relationship to the 'deletion' model of immunoglobulin gene rearrangement.免疫球蛋白可变区基因的图谱绘制：与免疫球蛋白基因重排的“缺失”模型的关系
Nucleic Acids Res. 1981 Nov 11;9(21):5725-35. doi: 10.1093/nar/9.21.5725.
10
Organization and expression of immunoglobulin genes in fetal liver hybridomas.胎儿肝脏杂交瘤中免疫球蛋白基因的组织与表达
Proc Natl Acad Sci U S A. 1981 Jan;78(1):247-51. doi: 10.1073/pnas.78.1.247.
Zotero 插件