一种用于检测血清中心肌肌钙蛋白 I 的荧光侧向流动免疫分析，用于在护理点进行肌肉损伤监测。

A Fluorescent Lateral Flow Immunoassay for the Detection of Skeletal Muscle Troponin I in Serum for Muscle Injury Monitoring at the Point of Care.

机构信息

The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.

Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, China.

出版信息

Biosensors (Basel). 2024 Aug 8;14(8):381. doi: 10.3390/bios14080381.

DOI:10.3390/bios14080381

PMID:39194610

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11352793/

Abstract

Exercise-induced muscle injury is one of the most common types of sports injuries. Skeletal muscle troponin I (skTnI) serves as an ideal biomarker in assessing such injuries, facilitating timely detection and evaluation. In this study, we develop a fluorescent sandwich lateral flow immunoassay (LFIA) combined with a desktop analyzer for rapid detection of skTnI. Through optimizing the reaction system, the assay achieves a satisfying detection performance, reaching a limit of detection (LOD) of 0.5 ng/mL with a turnaround time of 15 min. The proposed detection platform offers portability, ease of use, and high sensitivity, which facilitates the monitoring of exercise-induced muscle injuries at the point of care. This feature is particularly advantageous for end users, enabling timely detection of sports-related injuries and ultimately enhancing prognosis and sports life.

摘要

运动性肌肉损伤是最常见的运动损伤类型之一。骨骼肌肌钙蛋白 I（skTnI）作为评估此类损伤的理想生物标志物，有助于及时进行检测和评估。在这项研究中，我们开发了一种荧光三明治侧向流动免疫分析（LFIA），结合台式分析仪，用于快速检测 skTnI。通过优化反应体系，该分析方法实现了令人满意的检测性能，检测限（LOD）为 0.5ng/mL，检测时间为 15 分钟。该检测平台具有便携性、易用性和高灵敏度，有助于在护理点监测运动性肌肉损伤。对于终端用户来说，这一特点尤其有利，能够及时发现与运动相关的损伤，从而改善预后和运动寿命。