Department of Mechanical Engineering, Chung Yuan Christian University, Chung Li District, Taoyuan City 320314, Taiwan.
Department of Emergency and Critical Care Medicine, Changhua Christian Hospital, Changhua 50006, Taiwan.
Biosensors (Basel). 2024 Aug 9;14(8):386. doi: 10.3390/bios14080386.
Due to the clinical similarities between pulmonary embolism (PE) and myocardial infarction (MI), physicians often encounter challenges in promptly distinguishing between them, potentially missing the critical window for the correct emergency response. This paper presents a biosensor, termed the PEMI biosensor, which is designed for the identification and quantitative detection of pulmonary embolism or myocardial infarction. The surface of the working electrode of the PEMI biosensor was modified with graphene oxide and silk fibroin to immobilize the mixture of antibodies. Linear sweep voltammetry was employed to measure the current-to-potential mapping of analytes, with the calculated curvature serving as a judgment index. Experimental results showed that the curvature exhibited a linear correlation with the concentration of antigen FVIII, and a linear inverse correlation with the concentration of antigen cTnI. Given that FVIII and cTnI coexist in humans, the upper and lower limits were determined from the curvatures of a set of normal concentrations of FVIII and cTnI. An analyte with a curvature exceeding the upper limit can be identified as pulmonary embolism, while a curvature falling below the lower limit indicates myocardial infarction. Additionally, the further the curvature deviates from the upper or lower limits, the more severe the condition. The PEMI biosensor can serve as an effective detection platform for physicians.
由于肺栓塞 (PE) 和心肌梗死 (MI) 在临床上具有相似性，医生在快速区分它们时经常会遇到挑战，可能会错过正确紧急反应的关键窗口期。本文提出了一种生物传感器，称为 PEMI 生物传感器，用于识别和定量检测肺栓塞或心肌梗死。PEMI 生物传感器的工作电极表面经过氧化石墨烯和丝素蛋白修饰，以固定抗体混合物。线性扫描伏安法用于测量分析物的电流-电位映射，计算出的曲率作为判断指标。实验结果表明，曲率与抗原 FVIII 的浓度呈线性相关，与抗原 cTnI 的浓度呈线性反比关系。由于 FVIII 和 cTnI 在人体内共存，通过一组正常浓度的 FVIII 和 cTnI 的曲率确定了上限和下限。曲率超过上限的分析物可被识别为肺栓塞，而低于下限的曲率则表明心肌梗死。此外，曲率偏离上限或下限的程度越大，病情越严重。PEMI 生物传感器可以作为医生的有效检测平台。
