Devanthi Putu Virgina Partha, Pratama Ferren, Pramanda Ihsan Tria, Bani Mario Donald, Kadar Adinda Darwati, Kho Katherine
Indonesia International Institute for Life Sciences, Pulomas Barat Kavling 88, Jakarta 13210, Indonesia.
J Fungi (Basel). 2024 Aug 7;10(8):555. doi: 10.3390/jof10080555.
Mycoprotein is an alternative protein produced through fungal fermentation. However, it typically relies on refined glucose syrup derived from starch, which can be costly and unsustainable. This study investigates the potential of soybean processing by-products (okara and soy whey) as alternative substrates for producing mycoprotein using . was cultured for 7 days at 30 °C in diluted okara (1:50) and soy whey (1:1) with or without agitation (100 rpm). Soy whey produced higher biomass yields (369.2-408.8 mg dry biomass/g dry substrate), but had a lower biomass concentration (0.783-0.867 g dry weight/L). Conversely, okara produced a higher biomass concentration (2.02 g dry weight/L) with a yield of 114.7 mg dry biomass/g dry substrate. However, biomass formation in okara was only observed in static conditions, as agitation caused biomass to entangle with soy pulp, hampering its production. Additionally, okara tended to release protein into the media, while soy whey accumulated protein within the biomass, reaching up to 53% / protein content. The results of this study provide a promising approach to addressing both soybean processing waste reduction and food security concerns.
真菌蛋白是通过真菌发酵产生的一种替代蛋白质。然而，它通常依赖于由淀粉衍生而来的精制葡萄糖浆，这可能成本高昂且不可持续。本研究调查了大豆加工副产物（豆渣和大豆乳清）作为使用 生产真菌蛋白的替代底物的潜力。 在30°C下于稀释的豆渣（1:50）和大豆乳清（1:1）中培养7天，有或无搅拌（100转/分钟）。大豆乳清产生了更高的生物量产量（369.2 - 408.8毫克干生物量/克干底物），但生物量浓度较低（0.783 - 0.867克干重/升）。相反，豆渣产生了更高的生物量浓度（2.02克干重/升），产量为114.7毫克干生物量/克干底物。然而，豆渣中的生物量形成仅在静态条件下观察到，因为搅拌会导致生物量与大豆浆缠结，阻碍其生产。此外，豆渣倾向于将蛋白质释放到培养基中，而大豆乳清则在生物量中积累蛋白质，蛋白质含量高达53%。本研究结果为解决大豆加工废弃物减少和粮食安全问题提供了一种有前景的方法。
