Lv Wuyun, Tu Yiyi, Xu Ting, Zhang You, Chen Junjie, Yang Nan, Wang Yuchun
College of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou 311300, China.
The People's Government Office of Bengbu City, Bengbu 233000, China.
J Fungi (Basel). 2024 Aug 16;10(8):579. doi: 10.3390/jof10080579.
The mitochondrial distribution and morphology family 33 gene () regulates mitochondrial homeostasis by mediating the mitochondrial fission process in yeast. The wheat head blight contains an FgMdm33 protein that is orthologous to Mdm33, albeit its function remains unknown. We have reported here the roles of FgMdm33 in regulating fungal morphogenesis, mitochondrial morphology, autophagy, apoptosis, and fungal pathogenicity. The Δ mutants generated through a homologous recombination strategy in this study exhibited defects in terms of mycelial growth, conidia production, and virulence. Hyphal cells lacking displayed elongated mitochondria and a dispensable respiratory-deficient growth phenotype, indicating the possible involvement of in mitochondrial fission. The Δ mutants displayed a remarkable reduction in the proteolysis of GFP-FgAtg8, whereas the formation of autophagic bodies in the hyphal cells of mutants was recorded under the induction of mitophagy. In addition, the transcriptional expression of the apoptosis-inducing factor 1 gene () was significantly upregulated in the Δ mutants. Cumulatively, these results indicate that is involved in mitochondrial fission, non-selective macroautophagy, and apoptosis and that it regulates fungal growth, conidiation, and pathogenicity of the head blight pathogen.
线粒体分布与形态家族33基因（）通过介导酵母中的线粒体分裂过程来调节线粒体稳态。小麦赤霉病菌含有一种与Mdm33直系同源的FgMdm33蛋白，尽管其功能尚不清楚。我们在此报告了FgMdm33在调节真菌形态发生、线粒体形态、自噬、凋亡及真菌致病性方面的作用。本研究通过同源重组策略产生的Δ突变体在菌丝生长、分生孢子产生和毒力方面表现出缺陷。缺乏的菌丝细胞显示出线粒体延长和一种可有可无的呼吸缺陷生长表型，表明可能参与线粒体分裂。Δ突变体在GFP-FgAtg8的蛋白水解方面显著降低，而在诱导线粒体自噬的情况下，记录到突变体菌丝细胞中自噬体的形成。此外，凋亡诱导因子1基因（）的转录表达在Δ突变体中显著上调。累积这些结果表明参与线粒体分裂、非选择性巨自噬和凋亡，并且它调节赤霉病菌的真菌生长、产孢和致病性。
