Li Wenya, Chen Hua, Cheng Jianhu, Zhang Min, Xu Yan, Wang Lihua, Zhao Xueqiao, Zhang Jinyao, Liu Bangdi, Sun Jing
School of Architecture and Art, Hebei University of Engineering, Handan 056038, China.
Academy of Agricultural Planning and Engineering, Ministry of Agriculture and Rural Affairs, Beijing 100125, China.
Metabolites. 2024 Jul 29;14(8):417. doi: 10.3390/metabo14080417.
This study aimed to explore the effects of GSBa-1 treatment on anthracnose disease resistance and the metabolism of reactive oxygen species (ROS) and phenylpropanoids in mangoes during storage. Mangoes were soaked in a solution containing 1 × 10 CFU/mL of GSBa-1. The anthracnose disease incidence, disease index, respiration intensity, ethylene release, reactive oxygen species content, and the activities of related metabolic enzymes, phenylpropanoid-related metabolic enzymes, and phenolic acids in the skin and pulp of mangoes were investigated under normal temperature storage conditions. The results showed that the antagonistic bacterial treatment (ABT) did not significantly inhibit the growth of in vitro. However, it significantly reduced the incidence of mango anthracnose disease when applied to the mango peel. ABT enhanced the latent resistance of mango to anthracnose disease by activating its reactive oxygen and phenylpropanoid metabolism. It maintained higher levels of ROS production and elimination in the peel. Moreover, it rapidly activated manganese superoxide dismutase, induced the accumulation of HO, and enhanced the activity of manganese superoxide dismutase, catalase, ascorbate peroxidase, and peroxidase in the mango peel. Furthermore, ABT activated phenylalanine ammonia-lyase, cinnamic acid-4-hydroxylase, 4-coumaroyl-CoA ligase, and cinnamyl alcohol dehydrogenase in the mango peel and pulp, promoting the accumulation of antifungal phenolic acids such as gallic acid, catechins, and ellagic acid. GSBa-1 may be a potent inhibitor of mango anthracnose, primarily enhancing the resistance of mangoes to anthracnose by synergistically activating ROS in the peel and phenylpropanoid metabolism in the pulp, thereby reducing the incidence of anthracnose effectively.
本研究旨在探讨GSBa-1处理对芒果贮藏期间炭疽病抗性以及活性氧（ROS）和苯丙烷类代谢的影响。将芒果浸泡在含有1×10 CFU/mL GSBa-1的溶液中。在常温贮藏条件下，研究了芒果炭疽病发病率、病情指数、呼吸强度、乙烯释放量、活性氧含量以及芒果果皮和果肉中相关代谢酶、苯丙烷类相关代谢酶和酚酸的活性。结果表明，拮抗细菌处理（ABT）在体外并未显著抑制其生长。然而，将其应用于芒果果皮时，可显著降低芒果炭疽病的发病率。ABT通过激活芒果的活性氧和苯丙烷类代谢，增强了芒果对炭疽病的潜在抗性。它在果皮中维持了较高水平的ROS产生和清除。此外，它迅速激活了锰超氧化物歧化酶，诱导了HO的积累，并增强了芒果果皮中锰超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶和过氧化物酶的活性。此外，ABT激活了芒果果皮和果肉中的苯丙氨酸解氨酶、肉桂酸-4-羟化酶、4-香豆酰辅酶A连接酶和肉桂醇脱氢酶，促进了没食子酸、儿茶素和鞣花酸等抗真菌酚酸的积累。GSBa-1可能是一种有效的芒果炭疽病抑制剂，主要通过协同激活果皮中的ROS和果肉中的苯丙烷类代谢来增强芒果对炭疽病的抗性，从而有效降低炭疽病的发病率。
