Liu Zongling, Ouyang Qiufei, Yi Sangui, Zhou Yuting, Xie Xiaona, Zhu Zhengjie
Guangxi Key Laboratory of Biology for Mango, Baise University, Baise, 533000, China.
School of Basic Medical Sciences, Youjiang Medical University for Nationalities, Baise, 533000, China.
BMC Genomics. 2025 Apr 29;26(1):411. doi: 10.1186/s12864-025-11631-9.
Mango anthracnose is a major biotic stress that limits mango production worldwide. An integrated transcriptome, small RNA (sRNA), and degradome sequencing analysis was conducted to determine the complex molecular mechanisms of mango anthracnose resistance. The transcriptome sequencing of 12 samples from a mango anthracnose resistant genotype under control and stress conditions on the 3rd and 5th day post inoculation identified 3,462 and 8,341 differentially expressed genes (DEGs), respectively, which were mainly involved in transcription factor activity, defense response, an obsolete oxidation - reduction process, and flavonoid biosynthetic processes. The sRNA sequencing of the samples identified 372 known and 104 novel miRNAs. A total of 81 differentially expressed miRNAs were identified, of which three were differentially expressed at both the 3rd and 5th day post-inoculation (dpi), including ath-MIR166e-p5_1ss15AC, mtr-miR156e, and csi-miR3954_L + 1_2ss17CG21CT. According to degradome sequencing, 2,274 targets were predicted for 341 miRNAs. The combined analysis of transcriptome and sRNA sequencing identified 257 miRNA-mRNA interaction pairs. In these pairs, csi-miR3954_L + 1_2ss17CG21CT was up-regulated at both the 3rd and 5th dpi under stress, which could cleave multiple sites of an NAC gene (LOC123212502) that was down-regulated under stress. Overall, these miRNAs and genes provide a molecular foundation for the miRNA-mediated response to mango anthracnose stress and can be regarded as promising candidates for mango improvement.
芒果炭疽病是一种严重的生物胁迫，限制了全球芒果产量。为了确定芒果抗炭疽病的复杂分子机制，进行了转录组、小RNA（sRNA）和降解组测序分析。对芒果炭疽病抗性基因型在接种后第3天和第5天的对照和胁迫条件下的12个样本进行转录组测序，分别鉴定出3462个和8341个差异表达基因（DEG），这些基因主要参与转录因子活性、防御反应、一个过时的氧化还原过程和类黄酮生物合成过程。对样本进行sRNA测序，鉴定出372个已知的和104个新的miRNA。共鉴定出81个差异表达的miRNA，其中3个在接种后第3天和第5天都有差异表达，包括ath-MIR166e-p5_1ss15AC、mtr-miR156e和csi-miR3954_L+1_2ss17CG21CT。根据降解组测序，预测341个miRNA有2274个靶标。转录组和sRNA测序的联合分析鉴定出257个miRNA-mRNA相互作用对。在这些对中，csi-miR3954_L+1_2ss17CG21CT在胁迫下接种后第3天和第5天都上调，它可以切割一个在胁迫下下调的NAC基因（LOC123212502）的多个位点。总体而言，这些miRNA和基因为miRNA介导的对芒果炭疽病胁迫的反应提供了分子基础，可被视为芒果改良的有希望的候选者。
