Geng Dongjie, Sun Yiqun, Liu Shouzan, Chen Wen, Gao Fei, Bai Yan, Zhang Shaobo
Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Hangzhou 311300, China.
College of Food and Health, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.
Metabolites. 2024 Aug 2;14(8):427. doi: 10.3390/metabo14080427.
Smith var. (Franch.) Hara is a medicinal plant that belongs to the Liliaceae family. Its main components are parissaponins, which have excellent medicinal effects such as anti-inflammatory, anti-tumor, etc. Improving the quality of parissaponins through artificial directional regulation has emerged as a practice to meet medical demand and is a new research hotspot. In this paper, plants were treated with UVA, UVB, and UVC, and the contents of PolyPhyllin VI (PPVI) and PolyPhyllin VII (PPVII), saponin synthase (squalene synthase, SS; cycloartenol synthase, CAS; cytochrome P450, CYP450; and glycosyl transferases, GT) activity, MDA, and the photosynthetic pigment indexes were measured and analyzed. The results showed that PPVII content increased by 32.43% with UVC treatment after 4 h (3.43 mg/g), but the PPVI and PPVII contents in the other groups decreased compared with CK (control group) and they did not return to the original level after 4 h. SS, CAS, CYP450, and GT synthases were activated in varying degrees via UV treatment and increased, respectively, by 22.93%, 10.83%, 20.15%, and 25.98%. Among them, GT, as the last of the synthetases, had a shorter response time to UVB (30 min) and UVC (15 min); the difference was sensible compared with CK. Moreover, UV had a stressing effect and promoted the rapid accumulation of MDA content (increased 17.66%, 34.53%, and 9.65%) and carotenoid (increased 7.58, 5.60, and 7.76 times) within 4 h compared to CK. UVB and UVC radiation visibly improved chlorophyll a content (42.56% and 35.45%), but UVA did not, and the change in chlorophyll b content showed no overt statistical difference. In addition, PPVI and PPVII were negatively correlated with SS, CAS, carotenoids, and MDA ( < 0.05) and positively correlated with CYP450, GT, and chlorophyll a ( < 0.05). This study provides a theoretical basis for using UV light to regulate secondary metabolism in , which is of great value for production management.
七叶一枝花变种（法瑞）原田是一种属于百合科的药用植物。其主要成分是重楼皂苷，具有抗炎、抗肿瘤等优异的药用功效。通过人工定向调控提高重楼皂苷的质量已成为满足医疗需求的一种实践，并且是一个新的研究热点。在本文中，用UVA、UVB和UVC处理植物，并测定和分析了重楼皂苷VI（PPVI）和重楼皂苷VII（PPVII）的含量、皂苷合成酶（鲨烯合酶，SS；环阿屯醇合酶，CAS；细胞色素P450，CYP450；糖基转移酶，GT）活性、丙二醛（MDA）以及光合色素指标。结果表明，经UVC处理4小时后，PPVII含量增加了32.43%（达到3.43毫克/克），但其他组的PPVI和PPVII含量与CK（对照组）相比有所下降，且4小时后未恢复到初始水平。经紫外线处理后，SS、CAS、CYP450和GT合成酶均有不同程度的激活，分别增加了22.93%、10.83%、20.15%和25.98%。其中，GT作为最后一个合成酶，对UVB（30分钟）和UVC（15分钟）的响应时间较短；与CK相比差异明显。此外，紫外线具有胁迫作用，与CK相比，在4小时内促进了MDA含量的快速积累（分别增加了17.66%、34.53%和9.65%）以及类胡萝卜素的积累（分别增加了7.58、5.60和
