Gu Xueling, Liao Simeng, Li Meng, Wang Jing, Tan Bie
Key Laboratory of Hunan Province for the Products Quality Regulation of Livestock and Poultry, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
Yuelushan Laboratory, Changsha 410128, China.
Vet Sci. 2024 Jul 25;11(8):333. doi: 10.3390/vetsci11080333.
Our previous study demonstrated that moderate inhibition of intestinal autophagy was beneficial to alleviate early weaning stress in piglets, but the detailed mechanism behind this was unclear. Microbiota-mediated enterocyte autophagy helps maintain intestinal homeostasis. This study investigated the effects of inhibition or activation of autophagy in intestinal microbial community compositions and metabolite profiles in piglets. Eighteen 24-day-old weaned piglets were divided into three groups (each treatment of six piglets) and treated daily with rapamycin (RAPA), chloroquine (CQ) or a control volume of normal saline (CON group). Before the formal trial, the piglets were allowed to acclimatize for 3 days, and then the trial period was 14 days. Collected samples from the ileum and colon underwent 16S rRNA gene sequencing and metabolite analysis. Significant differences in microbial composition were observed in both the ileum and colon of the RAPA and CQ groups compared to the CON group ( < 0.05). In addition, the relative levels of abundance of , , , and in the ileum and , , , , , , , and in the colon were improved in piglets fed the RAPA diet, whereas the relative levels of abundance of , and in the ileum and and in the colon were enhanced in the CQ group ( < 0.05). Meanwhile, metabolomic analysis showed that there were significant differences in metabolites among all groups ( < 0.05), and KEGG enrichment analysis revealed that differential metabolites were mainly enriched in the ABC transporters and biosynthesis of amino acids pathways. Furthermore, these metabolites were closely related to differential microorganisms ( < 0.05). Overall, autophagy inhibition regulates the composition of intestinal microorganisms and their metabolites, and these differential metabolites are significantly correlated with differential intestinal microorganisms, which may in turn affect the production performance of weaned piglets.
我们之前的研究表明，适度抑制肠道自噬有利于缓解仔猪早期断奶应激，但其背后的详细机制尚不清楚。微生物群介导的肠上皮细胞自噬有助于维持肠道稳态。本研究调查了抑制或激活自噬对仔猪肠道微生物群落组成和代谢物谱的影响。将18头24日龄断奶仔猪分为三组（每组六头仔猪），每天分别用雷帕霉素（RAPA）、氯喹（CQ）或等量生理盐水（CON组）处理。在正式试验前，让仔猪适应3天，然后试验期为14天。收集回肠和结肠样本进行16S rRNA基因测序和代谢物分析。与CON组相比，RAPA组和CQ组回肠和结肠的微生物组成均存在显著差异（<0.05）。此外，饲喂RAPA日粮的仔猪回肠中、、、和的相对丰度水平以及结肠中、、、、、、、和的相对丰度水平有所提高，而CQ组回肠中、和的相对丰度水平以及结肠中和的相对丰度水平有所增强（<0.05）。同时，代谢组学分析表明，所有组之间的代谢物存在显著差异（<0.05），KEGG富集分析显示差异代谢物主要富集在ABC转运蛋白和氨基酸生物合成途径中。此外，这些代谢物与差异微生物密切相关（<0.05）。总体而言，自噬抑制调节肠道微生物及其代谢物的组成，这些差异代谢物与差异肠道微生物显著相关，进而可能影响断奶仔猪的生产性能。
