School of Materials Science and Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, China.
Inner Mongolia Key Laboratory of Advanced Ceramic Materials and Devices, Inner Mongolia University of Science and Technology, Baotou, 014010, China.
Anal Sci. 2024 Dec;40(12):2181-2190. doi: 10.1007/s44211-024-00655-y. Epub 2024 Aug 28.
A straightforward label-free colorimetric aptasensor utilizing the aptamer-enhanced peroxidase-like activity of cobalt oxyhydroxide (CoOOH) nanosheets has been established for kanamycin detection. In the kanamycin-free state, aptamers adsorb onto the CoOOH surface through electrostatic forces, enhancing the peroxidase-like activity of CoOOH and thereby resulting in a strong absorption signal and a yellow hue in 3,3',5,5'-tetramethylbenzidine (TMB) upon termination of the reaction with a stop solution. Conversely, upon the introduction of kanamycin, aptamers and CoOOH nanosheets compete for binding to kanamycin, resulting in a significant decrease in the number of aptamers bound to CoOOH. As a result, the activity of CoOOH diminishes, leading to a corresponding reduction in coloration and absorbance of the solution. Hence, the quantitative determination of kanamycin could be realized by analyzing the absorbance variations. Under optimal conditions, the aptasensor demonstrated high sensitivity and specificity, with a linear detection range from 500 nM to 5 µM and a detection limit as low as 54.6 nM. Moreover, the aptasensor effectively identified kanamycin in river water samples, achieving a recovery rate between 91.7% and 102.1%. This approach offers good practicability and provides a novel platform for kanamycin detection in environmental samples.
一种简单的无标记比色适体传感器，利用钴氢氧化物（CoOOH）纳米片的适体增强过氧化物酶样活性，已被建立用于检测卡那霉素。在无卡那霉素的状态下，适体通过静电力吸附在 CoOOH 表面上，增强了 CoOOH 的过氧化物酶样活性，从而导致在反应终止时与终止溶液一起产生强烈的吸收信号和 3,3',5,5'-四甲基联苯胺（TMB）的黄色色调。相反，当引入卡那霉素时，适体和 CoOOH 纳米片竞争与卡那霉素结合，导致与 CoOOH 结合的适体数量显著减少。因此，CoOOH 的活性降低，导致溶液的着色和吸光度相应降低。因此，可以通过分析吸光度变化来实现卡那霉素的定量测定。在最佳条件下，该适体传感器表现出高灵敏度和特异性，线性检测范围为 500 nM 至 5 µM，检测限低至 54.6 nM。此外，该适体传感器有效地识别了河水中的卡那霉素，回收率在 91.7%至 102.1%之间。该方法具有良好的实用性，为环境样品中卡那霉素的检测提供了一个新的平台。
