Zhang Xuxin, Li Yihao, Xia Shaojie, Yang Zhenyuan, Zhang Baiyun, Wang Yonghong
Hunan Provincial Key Laboratory for Forestry Biotechnology and International Cooperation, Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha, 410004, China.
Yuelushan Laboratory, Changsha, 410004, China.
Anal Sci. 2025 Jan;41(1):45-53. doi: 10.1007/s44211-024-00672-x. Epub 2024 Sep 17.
Kanamycin (KAN) is widely used as a growth hormone analog and an antibacterial agent. However, abuse of this substance has resulted in the accumulation of excessive residue levels in foods of animal origin, which presents a significant risk to human health. A chemiluminescent aptasensor was constructed for the rapid quantitative detection of KAN by combining the properties of CoO nanoparticles (CoO NPs) nanozyme activity and DNA aptamer with high specificity. The DNA aptamer/CoO NPs nanozyme regulated the chemiluminescence signal by exploiting the chemiluminescent properties of luminol oxidation by HO. Specific binding of KAN to the aptamer led to the formation of a steric hindrance block in the solution, which inhibited the activity of nanozyme and reduced signal intensity. The degree of signal reduction is related to the concentration of KAN. Under optimal conditions, there was good linearity between KAN concentration and chemiluminescence signal intensity in the range of 0.5-8.0 μΜ, with a detection limit of 0.26 μΜ. The detection system performed well in the presence of competing antibiotics and was virtually unaffected. The method was also suitable for the detection of KAN in milk samples with sample recoveries of 97.8%-99.1%. The chemiluminescence sensor has the advantages of low cost, specificity, and sensitivity, and does not require an external light source or modification of the nucleic acid aptamer which makes it a promising candidate for applications in the field of food detection.
卡那霉素（KAN）被广泛用作生长激素类似物和抗菌剂。然而，这种物质的滥用导致动物源性食品中残留水平过高，对人类健康构成重大风险。通过结合CoO纳米颗粒（CoO NPs）的纳米酶活性和具有高特异性的DNA适配体的特性，构建了一种用于快速定量检测KAN的化学发光适配体传感器。DNA适配体/CoO NPs纳米酶利用HO氧化鲁米诺的化学发光特性来调节化学发光信号。KAN与适配体的特异性结合导致溶液中形成空间位阻，抑制了纳米酶的活性并降低了信号强度。信号降低的程度与KAN的浓度有关。在最佳条件下，KAN浓度与化学发光信号强度在0.5 - 8.0 μΜ范围内具有良好的线性关系，检测限为0.26 μΜ。该检测系统在存在竞争抗生素的情况下表现良好，几乎不受影响。该方法也适用于牛奶样品中KAN的检测，样品回收率为97.8% - 99.1%。该化学发光传感器具有成本低、特异性强和灵敏度高的优点，并且不需要外部光源或对核酸适配体进行修饰，这使其成为食品检测领域应用的有前途的候选者。
