Drug Discovery and Development Division, Patanjali Research Foundation, Governed by Patanjali Research Foundation Trust, NH-58, Haridwar, Uttarakhand 249 405, India; Department of Allied and Applied Sciences, University of Patanjali, Patanjali Yog Peeth, Roorkee-Haridwar Road, Haridwar, Uttarakhand 249 405, India; Patanjali Yog Peeth (UK) Trust, 40 Lambhill Street, Kinning Park, Glasgow G411AU, UK.
Drug Discovery and Development Division, Patanjali Research Foundation, Governed by Patanjali Research Foundation Trust, NH-58, Haridwar, Uttarakhand 249 405, India.
J Pharm Biomed Anal. 2024 Dec 15;251:116444. doi: 10.1016/j.jpba.2024.116444. Epub 2024 Aug 23.
The remarkable biodiversity of medicinal plants worldwide highlights their significance in traditional and alternative medicine. Astavarga, a group of eight medicinal herbs from the Himalayan region of India, including Roscoea purpurea (commonly known as Kakoli), is esteemed in Ayurveda for its health-promoting and rejuvenating properties. In this comprehensive study, we aimed to develop and optimise robust UHPLC-MS/QToF (Ultra-high-performance liquid chromatography-mass spectrometry coupled with quadrupole time of flight) and GC-MS/MS (Gas chromatography-tandem mass spectrometry) methods to identify the phytochemicals in R. purpurea root hydromethanolic extract and essential oil. We also conducted a comparative assessment of supercritical fluid extraction and conventional solvent extraction methods for the first time in R. purpurea root, highlighting their relevance to the medicinal field. Using the UHPLC/MS-QToF method, we identified a total of fifty-six phytometabolites, while sixteen volatile constituents were discerned within the essential oil of R. purpurea by GC-MS/MS method. Among the volatile constituents, β-eudesmol (40.84 %), guaiac acetate (10.55 %), and γ-eudesmol (10.31 %) were emerged as the principal components. Our findings were further compared with the volatile constituents extracted via supercritical fluid extraction and conventional solvent extraction methods. Notably, our research unveiled the presence of a carotenoid metabolite, 15-methyl retinol, for the first time. Furthermore, our fatty acid analysis of the supercritical fluid extract revealed elevated levels of unsaturated fatty acids, particularly oleic and linoleic acids. The methods were validated in terms of system specificity also. The discovery of these well-recognised therapeutically active components in R. purpurea significantly enhances its potential, highlighting its unique profile among medicinal plants in the Himalayan region and its suitability for traditional Ayurveda.
藏药材全世界范围内具有显著的生物多样性，这突出了它们在传统和替代医学中的重要性。在印度喜马拉雅地区，有一种名为 Astavarga 的八种药用植物群，包括 Roscoea purpurea（通常称为 Kakoli），因其具有促进健康和恢复活力的特性而在阿育吠陀医学中备受推崇。在这项综合性研究中，我们旨在开发和优化强大的 UHPLC-MS/QToF（超高效液相色谱-质谱联用四极杆飞行时间）和 GC-MS/MS（气相色谱-串联质谱）方法，以鉴定 R. purpurea 根水-甲醇提取物和精油中的植物化学物质。我们还首次对 R. purpurea 根的超临界流体提取和常规溶剂提取方法进行了比较评估，突出了它们与药用领域的相关性。使用 UHPLC/MS-QToF 方法，我们总共鉴定出了 56 种植物代谢产物，而通过 GC-MS/MS 方法则在 R. purpurea 精油中鉴定出了 16 种挥发性成分。在挥发性成分中，β-桉叶醇（40.84%）、乙酸愈创木酯（10.55%）和γ-桉叶醇（10.31%）是主要成分。我们的研究结果还与通过超临界流体提取和常规溶剂提取方法提取的挥发性成分进行了比较。值得注意的是，我们首次发现了一种类胡萝卜素代谢产物，即 15-甲基视黄醇。此外，我们对超临界流体提取物的脂肪酸分析显示，不饱和脂肪酸，特别是油酸和亚油酸的含量较高。该方法在系统特异性方面也得到了验证。在 R. purpurea 中发现这些公认的治疗活性成分，显著提高了其潜力，突出了它在喜马拉雅地区药用植物中的独特特征及其在传统阿育吠陀医学中的适用性。
