Baba A, Kimoto M, Tatsuno T, Inoue T, Iwata H
Biochem Biophys Res Commun. 1985 Feb 28;127(1):283-8. doi: 10.1016/s0006-291x(85)80156-x.
The microvessels isolated from rat cerebral cortex has arachidonate lipoxygenase activity, which was not due to possible contamination of the platelets. The major product was identified to be 12-hydroxyeicosatetraenoic acid. After homogenization and sonication of the microvessel preparations, the lipoxygenase activity was recovered both in the membrane- and the cytosol-fractions, whereas that in the platelets was recovered in the cytosol fraction. Membrane-bound lipoxygenase of the microvessels has apparent Km value of 3.8 microM for arachidonic acid, which was corresponded to 1/5 of that in the platelet enzyme. Microvessel lipoxygenase was inhibited by nordihydroguaiaretic acid but not by indomethacin.
从大鼠大脑皮层分离出的微血管具有花生四烯酸脂氧合酶活性，这并非由于可能存在的血小板污染。主要产物被鉴定为12-羟基二十碳四烯酸。微血管制剂经匀浆和超声处理后，脂氧合酶活性在膜组分和胞质溶胶组分中均有恢复，而血小板中的脂氧合酶活性仅在胞质溶胶组分中恢复。微血管的膜结合脂氧合酶对花生四烯酸的表观Km值为3.8 microM，这相当于血小板酶的1/5。微血管脂氧合酶受去甲二氢愈创木酸抑制，但不受吲哚美辛抑制。
