School of Environmental Science and Engineering, Environmental Microbiomics Research Center, Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-Sen University, Guangzhou 510006, China.
School of Environmental Science and Engineering, Environmental Microbiomics Research Center, Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-Sen University, Guangzhou 510006, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-Sen University, Guangzhou 510006, China.
J Hazard Mater. 2024 Nov 5;479:135604. doi: 10.1016/j.jhazmat.2024.135604. Epub 2024 Aug 22.
Triclosan (TCS) and tetracycline (TC) as common antibacterial agents are frequently detected in the influent of wastewater treatment plants. The TCS and TC exposure patterns may determine their impacts on wastewater treatment microbiomes, on which information remains unknown. In this study, the impacts of sequential exposure of TCS and TC on methanogenic digestion microbiomes in upflow anaerobic sludge blanket (UASB) reactors were analyzed and compared with that of the same microbiomes being simultaneously exposed to TCS and TC. Results indicated that the UASB reactor 2 (MD2) with sequential TCS-TC exposure consistently demonstrated higher chemical oxygen demand (COD) removal efficiency (94.7 %). In contrast, in the MD1 reactor, COD removal efficiency decreased from 94.4 % to 82.7 % upon simultaneous exposure to TCS and TC. Accordingly, a 1.8 times higher enrichment of total antibiotic resistance genes (ARGs) was observed in MD1 relative to MD2. Using a dissimilarity-overlap approach, the MD2 microbiome with sequential exposure was predominantly mediated by deterministic factors in their community assembly (largely contributed by abundant and intermediate biospheres), resulting in microbial interaction networks with higher average clustering coefficients and shorter average path lengths, compared to the MD1 microbiomes. Our results could support sustainable management of TCS and TC contamination in wastewater treatment plants.
三氯生（TCS）和四环素（TC）作为常见的抗菌剂，经常在污水处理厂的进水口被检测到。TCS 和 TC 的暴露模式可能决定它们对废水处理微生物组的影响，但目前这方面的信息还不清楚。在本研究中，分析了 TCS 和 TC 顺序暴露对上流式厌氧污泥床（UASB）反应器中产甲烷消化微生物组的影响，并将其与同时暴露于 TCS 和 TC 的相同微生物组进行了比较。结果表明，顺序暴露于 TCS-TC 的 UASB 反应器 2（MD2）始终表现出更高的化学需氧量（COD）去除效率（94.7%）。相比之下，在同时暴露于 TCS 和 TC 的 MD1 反应器中，COD 去除效率从 94.4%下降到 82.7%。相应地，在 MD1 中观察到总抗生素抗性基因（ARGs）的富集程度比 MD2 高 1.8 倍。通过不相似性-重叠方法，顺序暴露的 MD2 微生物组主要由其群落组装中的确定性因素介导（主要由丰富和中间生物区系贡献），与 MD1 微生物组相比，其微生物相互作用网络具有更高的平均聚类系数和更短的平均路径长度。我们的研究结果可以为污水处理厂中 TCS 和 TC 污染的可持续管理提供支持。
