Matsuura Takanori, Komatsu Keiji, Suzumura Toshikatsu, Stavrou Stella, Juanatas Mary Lou, Park Wonhee, Ogawa Takahiro
Weintraub Center for Reconstructive Biotechnology, UCLA School of Dentistry, Los Angeles, USA.
Department of Periodontology, Graduated School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan.
J Prosthodont Res. 2025 Apr 14;69(2):249-258. doi: 10.2186/jpr.JPR_D_24_00071. Epub 2024 Aug 27.
The maintenance of peri-implant health relies significantly on the integrity of the peri-implant seal, particularly vulnerable at the interface between implant abutment and soft tissue. Early healing stages around implants involve cellular exposure to oxidative stress. This study aimed to investigate whether vacuum ultraviolet (VUV)-treated titanium augments the growth and functionality of human gingival fibroblasts while mitigating cellular stress.
Machined titanium plates underwent treatment with 172 nm VUV light for one minute, with untreated plates as controls. Human gingival fibroblasts were cultured on treated and untreated plates, and their behavior, growth, and functionality were assessed. Functionally impaired fibroblasts, treated with hydrogen peroxide, were also cultured on these titanium plates, and plate-to-plate transmigration ability was evaluated.
Fibroblasts on VUV-treated titanium exhibited a 50% reduction in intracellular reactive oxygen species production compared to controls. Additionally, glutathione, an antioxidant, remained undepleted in cells on VUV-treated titanium. Furthermore, the expression levels of inflammatory cytokines IL-1β and IL-8 decreased by 40-60% on VUV-treated titanium. Consequently, fibroblast attachment and proliferation doubled on VUV-treated titanium compared to those in the controls, leading to enhanced cell retention. Plate-to-plate transmigration assays demonstrated that fibroblasts migrated twice as far on VUV-treated surfaces compared to those in the controls. In particular, the transmigration ability, impaired in functionally impaired fibroblasts on the controls, was preserved on VUV-treated titanium.
VUV-treated titanium promotes the growth, function, and migration of human gingival fibroblasts by reducing cellular stress and enhancing antioxidative capacity. Notably, the transmigration ability significantly improved on VUV-treated titanium.
种植体周围组织健康的维持在很大程度上依赖于种植体周围封闭的完整性，种植体基台与软组织的界面处尤为脆弱。种植体周围的早期愈合阶段涉及细胞暴露于氧化应激。本研究旨在探讨真空紫外线（VUV）处理的钛是否能促进人牙龈成纤维细胞的生长和功能，同时减轻细胞应激。
将加工后的钛板用172nm的VUV光处理1分钟，未处理的钛板作为对照。将人牙龈成纤维细胞培养在处理过和未处理过的钛板上，评估其行为、生长和功能。用过氧化氢处理的功能受损的成纤维细胞也培养在这些钛板上，并评估板间迁移能力。
与对照组相比，VUV处理的钛板上的成纤维细胞内活性氧生成减少了50%。此外，抗氧化剂谷胱甘肽在VUV处理的钛板上的细胞中未被耗尽。此外，VUV处理的钛板上炎症细胞因子IL-1β和IL-8的表达水平降低了40%-60%。因此，与对照组相比，VUV处理的钛板上的成纤维细胞附着和增殖增加了一倍，导致细胞保留增强。板间迁移试验表明，与对照组相比，成纤维细胞在VUV处理的表面上迁移的距离是对照组的两倍。特别是，对照组中功能受损的成纤维细胞受损的迁移能力在VUV处理的钛板上得以保留。
VUV处理的钛通过减轻细胞应激和增强抗氧化能力，促进人牙龈成纤维细胞的生长、功能和迁移。值得注意的是，VUV处理的钛板上的迁移能力显著提高。
