Leto Leandra, Favari Claudia, Agosti Anna, Del Vecchio Lorenzo, Di Fazio Andrea, Bresciani Letizia, Mena Pedro, Guarrasi Valeria, Cirlini Martina, Chiancone Benedetta
Department of Food and Drug, University of Parma, Viale Parco Area delle Scienze 27/A, 43124 Parma, Italy.
Institute of Biophysics, National Research Council (CNR), Via Ugo La Malfa 153, 90146 Palermo, Italy.
Antioxidants (Basel). 2024 Jul 28;13(8):909. doi: 10.3390/antiox13080909.
The demand for bioactive secondary metabolites of natural origin is increasing every day. Micropropagation could be a strategy to respond more quickly to market demands, regardless of seasonality. This research aims to evaluate in vitro-grown plants of two hop varieties, namely Columbus and Magnum, as a potential source of bioactive compounds. The extracts were characterized in terms of total phenolic content by a Folin-Ciocalteu assay and antioxidant capacity by DPPH, ABTS, and FRAP assays. The bioactive compound profile of the extracts from both varieties was determined by using UPLC-ESI-QqQ-MS/MS. The results confirmed richness in (poly)phenols and other secondary metabolites of the in vitro-grown hop plantlets. Thirty-two compounds belonging to the major families of phytochemicals characteristic of the species were identified, and twenty-six were quantified, mainly flavonoids, including xanthohumol and isoxanthohumol, phenolic acids, as well as α- and β-acids. This study confirms the validity of in vitro-derived hop plantlets as source of bioactive compounds to be used in the nutraceutical, pharmaceutical, and food industries.
对天然来源的生物活性次生代谢产物的需求与日俱增。微繁殖可能是一种能更快速响应市场需求的策略，不受季节性影响。本研究旨在评估两个啤酒花品种，即哥伦布和玛格努姆，在体外培养的植株作为生物活性化合物潜在来源的情况。通过福林 - 西奥尔特法测定提取物的总酚含量，并通过DPPH、ABTS和FRAP法测定抗氧化能力。使用超高效液相色谱 - 电喷雾 - 串联四极杆质谱（UPLC - ESI - QqQ - MS/MS）确定两个品种提取物的生物活性化合物谱。结果证实了体外培养的啤酒花幼苗中（多）酚类和其他次生代谢产物的丰富性。鉴定出了属于该物种特征性主要植物化学物质家族的32种化合物，并对26种进行了定量，主要是黄酮类化合物，包括黄腐酚和异黄腐酚、酚酸以及α - 和β - 酸。本研究证实了体外培养的啤酒花幼苗作为生物活性化合物来源在营养保健品、制药和食品工业中的有效性。
