Department of Obstetrics and Gynecology, Faculty of Medicine, Saitama Medical University, Iruma, Moroyama 350-0495, Saitama, Japan.
Biomedical Research Center, Faculty of Medicine, Saitama Medical University, Iruma, Moroyama 350-0495, Saitama, Japan.
Biomolecules. 2024 Aug 16;14(8):1014. doi: 10.3390/biom14081014.
Decidualization denotes the morphological and biological differentiating process of human endometrial stromal cells (HESCs). Fatty acid pathways are critical for endometrial decidualization. However, the participation of fatty acids as an energy source and their role in endometrial decidualization have received little attention. To identify fatty acids and clarify their role in decidualization, we comprehensively evaluated free fatty acid profiles using liquid chromatography/Fourier transform mass spectrometry (LC/FT-MS). LC/FT-MS analysis detected 26 kinds of fatty acids in the culture medium of decidualized or un-decidualized HESCs. Only the production of octanoic acid, which is an essential energy source for embryonic development, was increased upon decidualization. The expressions of genes related to octanoic acid metabolism including ACADL, ACADM, and ACADS; genes encoding proteins catalyzing the first step of mitochondrial fatty acid beta-oxidation; and ACSL5 and ACSM5; genes encoding fatty acid synthesis proteins were significantly altered upon decidualization. These results suggest that decidualization promotes lipid metabolism, implying that decidualized HESCs require energy metabolism of the mitochondria in embryo implantation.
蜕膜化表示人子宫内膜基质细胞（HESCs）的形态和生物学分化过程。脂肪酸途径对子宫内膜蜕膜化至关重要。然而，脂肪酸作为能量来源的参与及其在子宫内膜蜕膜化中的作用却很少受到关注。为了鉴定脂肪酸并阐明其在蜕膜化中的作用，我们使用液相色谱/傅里叶变换质谱（LC/FT-MS）全面评估了游离脂肪酸谱。LC/FT-MS 分析检测到蜕膜化或未蜕膜化 HESCs 培养物中的 26 种脂肪酸。只有十八烷酸（胚胎发育的必需能量来源）的产生在蜕膜化时增加。与包括 ACADL、ACADM 和 ACADS 在内的与十八烷酸代谢相关的基因的表达；编码催化线粒体脂肪酸β-氧化第一步的蛋白质的基因；以及 ACSL5 和 ACSM5；编码脂肪酸合成蛋白的基因在蜕膜化时发生了明显改变。这些结果表明蜕膜化促进了脂质代谢，这意味着蜕膜化的 HESCs 在胚胎植入时需要线粒体的能量代谢。
