一种对双链DNA具有抗体活性的人源单克隆IgM的特性分析。
Characterization of a human monoclonal IgM with antibody activity to dsDNA.
作者信息
Fermand J P, Danon F, Brouet J C
出版信息
Clin Exp Immunol. 1985 Feb;59(2):467-74.
Abstract
We report here the characterization and fine specificity of a human monoclonal IgM lambda reacting with double stranded DNA. The purified IgM and its Fab fragment bind to DNA in the Farr or Crithidia lucilae assays as well as in an immunoenzymatic test. In the latter case, binding was inhibited by native DNA, by DNA alternative co-polymers and by some homopolymers only, but not by monodeoxyribonucleotides and ribonucleotides. Since cardiolipin and phosphatidic acid were good inhibitors, these data suggest that the monoclonal IgM reacts with the sugar phosphate backbone of deoxyribonucleic acids. Interestingly, the patient was affected with both Waldenström's macroglobulinaemia and pernicious anaemia without any symptoms of systemic lupus erythematosus.
摘要
我们在此报告一种与双链DNA反应的人源单克隆IgM λ的特性及精细特异性。纯化的IgM及其Fab片段在Farr试验、克氏锥虫试验以及免疫酶试验中均能与DNA结合。在后一种情况下，只有天然DNA、DNA交替共聚物和一些均聚物能抑制结合，而单脱氧核糖核苷酸和核糖核苷酸则不能。由于心磷脂和磷脂酸是良好的抑制剂，这些数据表明该单克隆IgM与脱氧核糖核酸的磷酸糖骨架发生反应。有趣的是，该患者同时患有华氏巨球蛋白血症和恶性贫血，而无任何系统性红斑狼疮的症状。
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