Chen Jiaxin, Wang Siwei, Yin Xuejiao, Duan Chunhui, Li Jinhui, Liu Yueqin, Zhang Yingjie
College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China.
Institute of Cereal and Oil Crops, Hebei Key Laboratory of Crop Cultivation Physiology and Green Production, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, China.
Animals (Basel). 2024 Aug 14;14(16):2344. doi: 10.3390/ani14162344.
Changes in physiological and biochemical parameters are crucial for the reproductive performance and health of perinatal ewes. This study investigated the temporal variations in feed intake, nutrient digestibility, serum parameters, and ruminal fermentation on days 21, 14, and 7 before lambing (Q21, Q14, and Q7) and days 3, 7, and 14 after lambing (H3, H7, and H14). The results showed that dry matter intake (DMI) and glucose (Glu) gradually decreased ( < 0.05) before lambing and increased ( < 0.05) after lambing. The digestibility of dry matter (DMD), crude protein (CPD), and acid detergent fiber (ADFD) increased ( < 0.05) before lambing, then decreased ( < 0.05) on day H3, and then increased ( < 0.05) on day H14. The rumen pH, NH-N, and triglycerides (TG) gradually increased ( < 0.05) before lambing and were higher ( < 0.05) on day Q7 than after lambing. The concentrations of acetate, butyrate, and total volatile fatty acids (T-VFA) were lower ( < 0.05) on day Q7 than those on days Q21 and Q14, then increased ( < 0.05) after lambing. Total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) concentrations gradually decreased ( < 0.05) in perinatal ewes. BHBA and NEFA concentrations were lower ( < 0.05) on day Q21 than those from days Q14 to H14. The rumen microbiota compositions were different ( < 0.05) in perinatal ewes, and , , and were positively correlated ( < 0.05) with DMI, Glu, acetate, propionate, and T-VFA, and negatively correlated ( < 0.05) with LDL-C. was negatively correlated ( < 0.05) with DMI, Glu, acetate, propionate, butyrate, and T-VFA, but positively correlated ( < 0.05) with rumen pH and LDL-C. In summary, the DMI, nutrient digestibility, rumen fermentation, and serum parameters changed during the perinatal period of ewes, and the changes in DMI, serum glucose, acetate, propionate, and T-VFA were related to the rumen bacteria.
生理和生化参数的变化对围产期母羊的繁殖性能和健康至关重要。本研究调查了产羔前21天、14天和7天（Q21、Q14和Q7）以及产羔后3天、7天和14天（H3、H7和H14）母羊的采食量、养分消化率、血清参数和瘤胃发酵的时间变化。结果表明，产羔前干物质采食量（DMI）和葡萄糖（Glu）逐渐降低（P<0.05），产羔后升高（P<0.05）。干物质消化率（DMD）、粗蛋白消化率（CPD）和酸性洗涤纤维消化率（ADFD）在产羔前升高（P<0.05），然后在H3天降低（P<0.05），随后在H14天升高（P<0.05）。瘤胃pH值、NH₃-N和甘油三酯（TG）在产羔前逐渐升高（P<0.05），且在Q7天高于产羔后（P<0.05）。Q7天的乙酸、丁酸和总挥发性脂肪酸（T-VFA）浓度低于Q21天和Q14天，产羔后升高（P<0.05）。围产期母羊的总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）浓度逐渐降低（P<0.05）。Q21天的β-羟基丁酸（BHBA）和非酯化脂肪酸（NEFA）浓度低于Q14天至H14天。围产期母羊的瘤胃微生物群组成不同（P<0.05），且[具体微生物名称未给出]与DMI、Glu、乙酸、丙酸和T-VFA呈正相关（P<0.05），与LDL-C呈负相关（P<0.05）。[具体微生物名称未给出]与DMI、Glu、乙酸、丙酸、丁酸和T-VFA呈负相关（P<0.05），但与瘤胃pH值和LDL-C呈正相关（P<0.05）。总之，母羊围产期的DMI、养分消化率、瘤胃发酵和血清参数发生了变化，且DMI、血清葡萄糖、乙酸、丙酸和T-VFA的变化与瘤胃细菌有关。
