Chen Jiaxin, Wang Siwei, Yin Xuejiao, Duan Chunhui, Li Jinhui, Liu Yue-Qin, Zhang Yingjie
College of Animal Science and Technology, Hebei Agricultural University, Baoding, China.
Hebei Key Laboratory of Crop Cultivation Physiology and Green Production, Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, China.
Front Vet Sci. 2024 Sep 27;11:1472334. doi: 10.3389/fvets.2024.1472334. eCollection 2024.
Pregnancy toxemia (PT) is a nutritional metabolic disease of ewes in late pregnancy. This study aimed to reveal the relationship between rumen microbiota and PT.
We selected 10 healthy ewes (CON) and 10 pregnancy toxemia ewes (PT) at 135 days of gestation according to the blood β-hydroxybutyrate (BHBA), glucose (Glu) concentrations and clinical symptoms. Blood and rumen fluid were collected before morning feeding to determine serum biochemical indices and rumen fermentation parameters. Total DNA of rumen fluid was extracted and the V3-V4 regions of 16S rRNA were amplified by PCR for high-throughput sequencing.
The results showed that the serum concentrations of Glu, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), uric acid (UA), creatinine (Cr), acetate, propionate, butyrate, and microbial crude protein (MCP) were decreased ( < 0.05) and the concentrations of BHBA, aspartate aminotransferase (AST), acetate to propionate ratio (A/P), and ammonia nitrogen (NH-N)were higher ( < 0.05) in PT ewes than those in CON ewes. 16S rRNA analysis showed the differences of β-diversity were observed in rumen microbiota between CON and PT ewes. At the phylum level, the relative abundance of Bacteroidota and Proteobacteria were higher ( < 0.01), while Firmicutes was lower ( < 0.01) in PT ewes. At the genus level, the relative , , , , , , and were lower ( < 0.01) in PT ewes. Meanwhile, the relative abundance of Oribacterium, Butyrivibrio, Ruminococcus, and Lachnospiraceae_AC2044_group were positively correlated ( < 0.01) with Glu, INS, acetate, propionate, and butyrate, and negatively correlated ( < 0.01) with BHBA, P, GC, AST, and A/P.
In conclusion, the decrease of , , and in the rumen of PT ewes reduced the concentrations of volatile fatty acids (acetate, propionate, and butyrate) and serum Glu, and increased BHBA concentration, indicating that the differences in rumen bacteria genera were related to pregnancy toxemia of ewes.
妊娠毒血症（PT）是妊娠后期母羊的一种营养代谢性疾病。本研究旨在揭示瘤胃微生物群与PT之间的关系。
根据血液β-羟基丁酸（BHBA）、葡萄糖（Glu）浓度及临床症状，在妊娠135天时选取10只健康母羊（CON）和10只患妊娠毒血症的母羊（PT）。在晨饲前采集血液和瘤胃液，以测定血清生化指标和瘤胃发酵参数。提取瘤胃液的总DNA，通过PCR扩增16S rRNA的V3-V4区域进行高通量测序。
结果显示，PT母羊血清中Glu、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、尿酸（UA）、肌酐（Cr）、乙酸、丙酸、丁酸和微生物粗蛋白（MCP）的浓度降低（<0.05），而BHBA、天冬氨酸转氨酶（AST）、乙酸与丙酸比值（A/P）和氨氮（NH-N）的浓度高于（<0.05）CON母羊。16S rRNA分析表明，CON和PT母羊瘤胃微生物群的β-多样性存在差异。在门水平上，PT母羊中拟杆菌门和变形菌门的相对丰度较高（<0.01），而厚壁菌门较低（<0.01）。在属水平上，PT母羊中[此处原文缺失部分属名]的相对丰度较低（<0.01）。同时，口腔杆菌属、丁酸弧菌属、瘤胃球菌属和毛螺菌科_AC2044_组的相对丰度与Glu、胰岛素、乙酸、丙酸和丁酸呈正相关（<0.01），与BHBA、磷、总胆固醇、AST和A/P呈负相关（<0.01）。
总之，PT母羊瘤胃中[此处原文缺失部分属名]的减少降低了挥发性脂肪酸（乙酸、丙酸和丁酸）和血清Glu的浓度，并增加了BHBA浓度，表明瘤胃细菌属的差异与母羊妊娠毒血症有关。
