Zhang Chuankai, Kong Xiaohui, Hou Peijun, Gao Tengyun, Zheng Huaijun, Li Wenqing, Fu Tong, Zhang Liyang
College of Animal Science and Technology, Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animals, Henan Agricultural University, Zhengzhou, China.
Gongzhuling Hefeng Ruminant Feed Co., Ltd, Gongzhuling, China.
Anim Biosci. 2025 Oct;38(10):2125-2135. doi: 10.5713/ab.24.0852. Epub 2025 May 19.
The study aimed to compare the growth performance, rumen fermentation, and rumen bacterial community of fattening Hu sheep fed either total mixed ration (TMR) or pelleted total mixed ration (PTMR) and to assess the feeding efficiency.
In a 58-day feeding experiment, forty-eight Hu sheep were randomly assigned to two groups (TMR and PTMR), with six pens per group and four sheep per pen. Body weight and feed intake were measured throughout the experiment to assess growth performance. On the final day of the experiment, rumen fluid was collected from sheep using a rumen fluid collector two hours post-feeding.
The sheep in the PTMR group exhibited significantly higher body weight (p<0.05) and average daily gain (p<0.01) compared to those in the TMR group, although the effect of PTMR on dry matter intake was not statistically significant (p>0.05). No statistically significant differences were observed in rumen fermentation parameters between the TMR and PTMR groups. However, analysis of rumen bacteria revealed that the Sob, Ace, Bootstrap, Shannon, and Chao indices were significantly lower in the PTMR group compared to the TMR group (p<0.05). Bacteroidetes and Firmicutes were the predominant bacteria in all groups, with the abundance of Succinivibrionaceae_UCG-001 significantly lower in the PTMR group. The relative abundance of Rikenellaceae_RC9_gut_group, NK4A214_group, and Streptococcus was significantly higher in the TMR group than in the PTMR group (p<0.05). Bacterial function prediction showed downregulation of the energy production and conversion pathway in the PTMR group (p<0.05). Correlation analysis indicated that norank_f_Bacteroidales_RF16 positively correlated with butyric acid (p<0.05), while Anaerovibrio negatively correlated with acetic acid, propionic acid, butyric acid, and total volatile fatty acids (p<0.05).
In summary, the results demonstrate that PTMR significantly enhances average daily gain in Hu sheep while maintaining rumen fermentation parameters, although accompanied by modifications in rumen bacterial community structure.
本研究旨在比较饲喂全混合日粮（TMR）或颗粒全混合日粮（PTMR）的育肥湖羊的生长性能、瘤胃发酵及瘤胃细菌群落，并评估饲养效率。
在一项为期58天的饲养试验中，48只湖羊被随机分为两组（TMR组和PTMR组），每组6个圈舍，每个圈舍4只羊。在整个试验过程中测量体重和采食量以评估生长性能。在试验的最后一天，在饲喂后两小时使用瘤胃液采集器从羊身上采集瘤胃液。
与TMR组相比，PTMR组的羊体重显著更高（p<0.05），平均日增重显著更高（p<0.01），尽管PTMR对干物质采食量的影响无统计学意义（p>0.05）。TMR组和PTMR组之间的瘤胃发酵参数未观察到统计学显著差异。然而，瘤胃细菌分析显示，PTMR组的Sob、Ace、Bootstrap、Shannon和Chao指数显著低于TMR组（p<0.05）。拟杆菌门和厚壁菌门是所有组中的主要细菌，PTMR组中琥珀酸弧菌科_UCG-001的丰度显著更低。TMR组中理研菌科_RC9肠道菌群、NK4A214菌群和链球菌的相对丰度显著高于PTMR组（p<0.05）。细菌功能预测显示PTMR组中能量产生和转化途径下调（p<0.05）。相关性分析表明，未分类_f_拟杆菌目_RF16与丁酸呈正相关（p<0.05），而厌氧弧菌与乙酸、丙酸、丁酸和总挥发性脂肪酸呈负相关（p<0.05）。
总之，结果表明，PTMR显著提高湖羊的平均日增重，同时维持瘤胃发酵参数，尽管伴随着瘤胃细菌群落结构的改变。
