Li Rongyang, Cao Yan, Wu Wangjun, Liu Honglin, Xu Shiyong
College of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing 210095, China.
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Animals (Basel). 2024 Aug 22;14(16):2434. doi: 10.3390/ani14162434.
N6-methyladenosine (m6A) is a crucial RNA modification affecting skeletal muscle development. Rhein, an anti-inflammatory extract, inhibits , a key demethylase in m6A metabolism. Our study showed that during muscle fiber formation, and expression increased while m6A levels decreased. After muscle injury, and expression initially rose but later fell, while m6A levels initially dropped and then recovered. Inhibition of by Rhein reduced MyHC and expression, indicating myoblast differentiation suppression. In a mouse model, Rhein decreased MyHC expression and muscle fiber cross-sectional area, delaying muscle regeneration. Rhein's ability to increase RNA m6A modification delays skeletal muscle remodeling post-injury, suggesting a new medicinal application for this plant extract.
N6-甲基腺苷（m6A）是一种影响骨骼肌发育的关键RNA修饰。大黄酸是一种抗炎提取物，可抑制m6A代谢中的关键去甲基化酶。我们的研究表明，在肌纤维形成过程中，该去甲基化酶的表达增加而m6A水平降低。肌肉损伤后，该去甲基化酶的表达最初上升但随后下降，而m6A水平最初下降然后恢复。大黄酸对该去甲基化酶的抑制作用降低了肌球蛋白重链（MyHC）和其他相关蛋白的表达，表明成肌细胞分化受到抑制。在小鼠模型中，大黄酸降低了MyHC表达和肌纤维横截面积，延缓了肌肉再生。大黄酸增加RNA m6A修饰的能力延迟了损伤后骨骼肌的重塑，提示这种植物提取物有新的药用价值。
