Polemis Michalis, Sideroglou Theologia, Chrysostomou Anthi, Mandilara Georgia D
Computational Biology Department, Central Public Health Laboratory, National Public Health Organization (EODY), 16672 Vari, Greece.
Directorate of Epidemiological Surveillance and Intervention for Infectious Diseases, National Public Health Organization (EODY), 15123 Maroussi, Greece.
Antibiotics (Basel). 2024 Jul 29;13(8):708. doi: 10.3390/antibiotics13080708.
subsp. serotype Enteritidis ( Enteritidis) is one of the major causes of foodborne infections and is responsible for many national and multi-country foodborne outbreaks worldwide. In Greece, human salmonellosis is a mandatory notifiable disease, with laboratory surveillance being on a voluntary basis. This study aims to provide the first insights into the genetic characteristics and antimicrobial resistance profiles of 47 Enteritidis human isolates using whole-genome sequencing (WGS) technology. The Enteritidis population was mainly resistant to fluoroquinolones due to point mutations, whereas one isolate presented a multi-resistant plasmid-mediated phenotype. ST11 was the most frequent sequence type, and phylogenetic analysis through the cgMLST and SNP methods revealed considerable genetic diversity. Regarding virulence factors, 8 out of the 24 known SPIs and C63PI were detected. Due to the observed variability between countries, it is of utmost importance to record the circulating Enteritidis strains' structure and genomic epidemiology at the national level. WGS is a valuable tool that is revolutionizing our approach to by providing a deeper understanding of these pathogens and their impact on human health.
肠炎亚种血清型肠炎沙门氏菌（肠炎沙门氏菌）是食源性感染的主要原因之一，在全球范围内导致了许多国家和多国的食源性疾病暴发。在希腊，人类沙门氏菌病是一种法定须上报的疾病，实验室监测是自愿进行的。本研究旨在利用全基因组测序（WGS）技术，首次深入了解47株肠炎沙门氏菌人类分离株的遗传特征和抗菌药物耐药谱。肠炎沙门氏菌群体主要因点突变而对氟喹诺酮类药物耐药，而有一株分离株呈现出多重耐药的质粒介导表型。ST11是最常见的序列型，通过cgMLST和SNP方法进行的系统发育分析显示出相当大的遗传多样性。关于毒力因子，在24种已知的SPI和C63PI中检测到了8种。由于各国之间观察到的变异性，在国家层面记录流行的肠炎沙门氏菌菌株的结构和基因组流行病学至关重要。WGS是一种有价值的工具，通过更深入地了解这些病原体及其对人类健康的影响，正在彻底改变我们应对[此处原文似乎不完整]的方法。
