Fernandez Sara Alonso, Pelaez-Prestel Hector F, Ras-Carmona Alvaro, Mozas-Gutierrez Juan, Reyes-Manzanas Raquel, Reche Pedro A
Department of Immunology & O2, Faculty of Medicine, Complutense University of Madrid, Pza Ramon y Cajal, S/N, 28040 Madrid, Spain.
Biomedicines. 2024 Aug 19;12(8):1885. doi: 10.3390/biomedicines12081885.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains a public health concern due to infections with new SARS-CoV-2 variants. Therefore, finding effective preventive and therapeutic treatments against all SARS-CoV-2 variants is of great interest. In this study, we examined the capacity of eucalyptus essential oil (EEO) and eucalyptol (EOL) to prevent SARS-CoV-2 infection, using as a model SARS-CoV-2 Spike pseudotyped lentivirus (SARS-CoV-2 pseudovirus) and 293T cells transfected with human angiotensin-converting enzyme 2 (hACE2-293T cells). First, we determined the cytotoxicity of EEO and EOL using the MTT colorimetric assay, selecting non-cytotoxic concentrations ≤ 0.1% (/) for further analysis. Subsequently, we evaluated the capacity of EEO and EOL in cell cultures to preclude infection of hACE2-293T cells by SARS-CoV-2 pseudovirus, using a luciferase-based assay. We found that EEO and EOL significantly reduced SARS-CoV-2 pseudovirus infection, obtaining IC values of 0.00895% and 0.0042% (/), respectively. Likewise, EEO and EOL also reduced infection by vesicular stomatitis virus (VSV) pseudovirus, although higher concentrations were required. Hence, EEO and EOL may be able to inhibit SARS-CoV-2 infection, at least partially, through a Spike-independent pathway, supporting the implementation of aromatherapy with these agents as a cost-effective antiviral measure.
由于新型严重急性呼吸综合征冠状病毒2（SARS-CoV-2）变种的感染，SARS-CoV-2仍然是一个公共卫生问题。因此，寻找针对所有SARS-CoV-2变种的有效预防和治疗方法备受关注。在本研究中，我们以SARS-CoV-2刺突蛋白假型慢病毒（SARS-CoV-2假病毒）为模型，并用转染了人血管紧张素转换酶2的293T细胞（hACE2-293T细胞），研究了桉叶精油（EEO）和桉叶油素（EOL）预防SARS-CoV-2感染的能力。首先，我们使用MTT比色法测定了EEO和EOL的细胞毒性，选择≤0.1%（/）的无细胞毒性浓度进行进一步分析。随后，我们使用基于荧光素酶的检测方法，评估了EEO和EOL在细胞培养物中阻止SARS-CoV-2假病毒感染hACE2-293T细胞的能力。我们发现，EEO和EOL显著降低了SARS-CoV-2假病毒感染，其半数抑制浓度（IC）值分别为0.00895%和0.0042%（/）。同样，EEO和EOL也降低了水泡性口炎病毒（VSV）假病毒的感染，尽管需要更高的浓度。因此，EEO和EOL可能至少部分通过不依赖刺突蛋白的途径抑制SARS-CoV-2感染，支持将这些药物用于芳香疗法作为一种经济有效的抗病毒措施。
