Renz Miriam, Siegert Pascal, Mohnke Katja, Ruemmler Robert, Frauenknecht Katrin, Sommer Clemens, Harder Anja
Department of Anesthesiology, University Medical Center of the Johannes Gutenberg University Mainz, 55131 Mainz, Germany.
Institute of Neuropathology, University Medical Center of the Johannes Gutenberg University Mainz, 55131 Mainz, Germany.
Biomedicines. 2024 Aug 19;12(8):1896. doi: 10.3390/biomedicines12081896.
Experimental animal studies of hypoxic-ischemic injury of the hippocampus of pigs are limited due to the unprecise definition of hippocampal subfields, cornu ammonis 1 to 4, compared to humans. Given that the pig model closely mirrors human physiology and serves as an important model for critical care research, a more precise description is necessary to draw valid conclusions applicable to human diseases. In our study, we were able to precisely define the CA2 and its adjacent regions in a domestic pig model by arginine vasopressin receptor 1B (AVPR1B) and calbindin-D28K like (CaBP-Li) expression patterns. Our findings demonstrate that the histoarchitecture of the porcine cornu ammonis subfields closely resembles that of the human hippocampus. Notably, we identified unusually strong neuronal damage in regions of the pig hippocampus following global ischemia, which are typically not susceptible to hypoxic-ischemic damage in humans.
与人类相比，由于对海马体亚区（即海马角1至4区）的定义不够精确，猪海马体缺氧缺血性损伤的实验动物研究受到限制。鉴于猪模型能紧密反映人类生理机能，且是危重症护理研究的重要模型，因此需要更精确的描述，以便得出适用于人类疾病的有效结论。在我们的研究中，我们能够通过精氨酸加压素受体1B（AVPR1B）和钙结合蛋白-D28K样蛋白（CaBP-Li）的表达模式，在家猪模型中精确界定CA2区及其相邻区域。我们的研究结果表明，猪海马角亚区的组织结构与人类海马体极为相似。值得注意的是，我们发现，在全脑缺血后，猪海马体区域出现了异常严重的神经元损伤，而这些区域在人类中通常不易受到缺氧缺血性损伤。
