Ono Yosuke, Kobayashi Yuta, Shimada Shigeki, Fukushi Yoshiyuki, Yoshino Osamu, Wada Shinichiro, Yamada Hideto
Department of Obstetrics and Gynecology, University of Yamanashi, 1110 Shimokawahigashi, Chuo 409-3898, Yamanashi, Japan.
Department of Obstetrics and Gynecology, Teine Keijinkai Hospital, 1-40, 12-chome, Maeda, Teine-ku, Sapporo 006-8555, Hokkaido, Japan.
J Clin Med. 2024 Aug 7;13(16):4605. doi: 10.3390/jcm13164605.
: This prospective study evaluated whether endometriosis is associated with chronic endometritis (CE) and affects the uterine endometrium microbiome (UEM) in women with repeated implantation failure (RIF). : Forty-three women with RIF were divided into 12 with endometriosis (EM) and 31 without endometriosis (non-EM). The UEM was examined by 16S ribosomal RNA (rRNA) sequencing, and CE was determined by CD 138 staining (plasma cells > 5.15/10 mm) simultaneously. : The EM group had a higher bacterial number (EM vs. non-EM; median [range], 6.5 vs. 3 [3-11, 1-16], = 0.009), while the frequency of species did not change. The rates of presence of (41.7% [5/12] vs. 3.3% [1/31], = 0.004) and species (58.3% [7/12] vs. 16.1% [5/31], = 0.017) were higher in the EM group. The prevalence of CE did not differ between the two groups. Multivariable logistic regression analysis revealed that the presence of species (odds ratio, 10.97, 95% confidence interval, 1.17-249.37, = 0.036) was associated with endometriosis. In the EM group, five women with species had a higher number of bacterial species (10 vs. 5 [6-11, 3-7], = 0.021) and higher Shannon diversity index (0.50 vs. 0.20 [0.19-1.39, 0.03-0.46], = 0.026) than seven without species. : and species, and the increased number of bacterial species in UEM may be related to the pathogenesis of RIF complicated by endometriosis.
本前瞻性研究评估了子宫内膜异位症是否与慢性子宫内膜炎（CE）相关，以及是否会影响反复种植失败（RIF）女性的子宫内膜微生物群（UEM）。43例RIF女性被分为12例患有子宫内膜异位症（EM）和31例未患有子宫内膜异位症（非EM）。通过16S核糖体RNA（rRNA）测序检测UEM，并同时通过CD 138染色（浆细胞>5.15/10 mm）确定CE。EM组细菌数量更高（EM组与非EM组；中位数[范围]，6.5对3[3 - 11，1 - 16]，P = 0.009），而物种频率未改变。EM组中普雷沃菌属（41.7%[5/12]对3.3%[1/31]，P = 0.004）和动弯杆菌属物种（58.3%[7/12]对16.1%[5/31]，P = 0.017）的存在率更高。两组之间CE的患病率无差异。多变量逻辑回归分析显示，动弯杆菌属物种的存在（比值比，10.97，95%置信区间，1.17 - 249.37，P = 0.036）与子宫内膜异位症相关。在EM组中，5例有动弯杆菌属物种的女性比7例没有该物种的女性具有更高的细菌物种数量（10对5[6 - 11，3 - 7]，P = 0.021）和更高的香农多样性指数（0.50对0.20[0.19 - 1.39，0.03 - 0.46]，P = 0.026）。普雷沃菌属和动弯杆菌属物种以及UEM中细菌物种数量的增加可能与子宫内膜异位症并发RIF的发病机制有关。
