二氯乙酸对灌注大鼠后肢支链氨基酸分解代谢的差异作用。
Differential effect of dichloroacetate on branched-chain amino acid catabolism in perfused rat hindlimbs.
作者信息
Schadewaldt P, Radeck W, Staib W
出版信息
FEBS Lett. 1985 Apr 8;183(1):33-6. doi: 10.1016/0014-5793(85)80948-0.
PMID:3920079
Abstract
The effect of 1 mM and 5 mM dichloroacetate on the catabolism of branched-chain amino acids in isolated rat hindlimbs was investigated in perfusions with 0.5 mM 1-14C-labeled L-leucine or L-valine. The results demonstrate an increasing effect of dichloroacetate on the flux through skeletal muscle branched-chain 2-oxo acid dehydrogenase. A minor effect was observed with the high dichloroacetate concentration. Evidence is presented that this was essentially due to diminished pyruvate supply.
摘要
在含有0.5 mM 1-¹⁴C标记的L-亮氨酸或L-缬氨酸的灌注实验中，研究了1 mM和5 mM二氯乙酸对分离的大鼠后肢支链氨基酸分解代谢的影响。结果表明，二氯乙酸对骨骼肌支链2-氧代酸脱氢酶通量的影响不断增加。高浓度二氯乙酸的影响较小。有证据表明，这主要是由于丙酮酸供应减少所致。
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