大鼠膈肌体外支链氨基酸代谢及丙氨酸生成。二氯乙酸的作用。
Branched-chain amino acid metabolism and alanine formation in rat diaphragm muscle in vitro. Effects of dichloroacetate.
作者信息
Snell K, Duff D A
出版信息
Biochem J. 1984 Nov 1;223(3):831-5. doi: 10.1042/bj2230831.
Abstract
Dichloroacetate (which activates pyruvate dehydrogenase) decreases the release of alanine, pyruvate and lactate in hemidiaphragm incubations with valine. Dichloroacetate interferes with alanine formation by diverting pyruvate into oxidative pathways, which not only limits pyruvate availability for direct transamination to form alanine but also indirectly affects branched-chain amino acid transamination by limiting 2-oxoglutarate regeneration from glutamate.
摘要
二氯乙酸（可激活丙酮酸脱氢酶）在与缬氨酸共同孵育的半膈肌实验中，可减少丙氨酸、丙酮酸和乳酸的释放。二氯乙酸通过将丙酮酸导入氧化途径来干扰丙氨酸的形成，这不仅限制了丙酮酸直接转氨生成丙氨酸的可用性，还通过限制从谷氨酸再生α-酮戊二酸而间接影响支链氨基酸的转氨作用。
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