Spindler-Vomachka M, Johnson D C
Diabetologia. 1985 Jan;28(1):38-44. doi: 10.1007/BF00276998.
Infertility associated with anovulation and loss of regular oestrous cyclicity is a consequence of diabetes mellitus in the rat. In an attempt to define loci of altered function, studies were undertaken to examine various aspects of hypothalamic-pituitary function in rats treated with streptozotocin. Medial basal hypothalamic fragments from adult female diabetic rats contained the same amount of gonadotrophin-releasing hormone but, with depolarization, released slightly but insignificantly (p greater than 0.05) more than did those from control animals. Furthermore, release of luteinizing hormone from pituitaries exposed to hypothalamic gonadotrophin-releasing hormone was not altered by diabetes. Removal of the negative feedback effect of gonadal steroids upon the hypothalamic-pituitary axis produced an increase in luteinizing hormone and follicle stimulating hormone concentrations in the serum of normal rats within 6h (p less than 0.05), whereas 24h were required for similar increases in diabetic rats. However, the same concentrations of gonadotrophins were found in diabetic and control animals 120 h after ovariectomy. The inhibitory action of oestradiol benzoate on the secretion of gonadotrophins was more pronounced in ovariectomized diabetic than in control rats. A 74% depression in serum luteinizing hormone (p less than 0.01) was produced by 0.5 microgram oestradiol benzoate per day in diabetic rats, while 5 micrograms was required in control animals. Similar reductions in follicle stimulating hormone concentrations (50%, p less than 0.05) were obtained by injecting 5 micrograms of the oestrogen into diabetic or 50 micrograms into control rats. Increases in serum prolactin were greater in the control animals however.(ABSTRACT TRUNCATED AT 250 WORDS)
无排卵和正常发情周期丧失相关的不育是大鼠糖尿病的一个后果。为了确定功能改变的位点，开展了研究以检查用链脲佐菌素处理的大鼠下丘脑 - 垂体功能的各个方面。成年雌性糖尿病大鼠的内侧基底下丘脑片段所含促性腺激素释放激素的量相同，但去极化时，其释放量比对照动物的片段略有增加，但不显著（p大于0.05）。此外，糖尿病并未改变垂体在接触下丘脑促性腺激素释放激素时促黄体生成素的释放。去除性腺类固醇对下丘脑 - 垂体轴的负反馈作用后，正常大鼠血清中促黄体生成素和促卵泡激素浓度在6小时内升高（p小于0.05），而糖尿病大鼠则需要24小时才有类似升高。然而，卵巢切除术后120小时，糖尿病大鼠和对照动物的促性腺激素浓度相同。苯甲酸雌二醇对促性腺激素分泌的抑制作用在去卵巢的糖尿病大鼠中比对照大鼠更明显。每天0.5微克苯甲酸雌二醇使糖尿病大鼠血清促黄体生成素降低74%（p小于0.01），而对照动物需要5微克。向糖尿病大鼠注射5微克该雌激素或向对照大鼠注射50微克，促卵泡激素浓度有类似降低（50%，p小于0.05）。然而，对照动物血清催乳素的升高幅度更大。（摘要截断于250字）
