Dudley S D, Ramirez I, Wade G N
Neuroendocrinology. 1981 Jul;33(1):1-6. doi: 10.1159/000123192.
Withdrawal of exogenous insulin for 24 h in ovariectomized, streptozotocin-diabetic rats significantly impairs estradiol uptake in whole homogenate fractions of hypothalamus-preoptic area and pituitary gland. Significant reductions in cell nuclear fractions from the same tissues are seen after 36 h of insulin deprivation. Subsequent reinstatement of insulin treatment does not yield full recovery of estradiol uptake after 24 h of insulin replacement. Fat content of the diet has no effect on brain or pituitary estradiol uptake in diabetic animals deprived of insulin for 36 h. Circulating levels of triglycerides, ketones, glucose, glycerol and free fatty acids were found to predict uptake levels to a significant extent, but no single metabolite is reliably predictive across tissues. These data demonstrate that insulin-dependent changes in brain and pituitary uptake of estradiol in rats are slow to develop, and they support the hypothesis that at least some of the reproductive dysfunctions observed in diabetic rats may be the result of impaired cell nuclear estradiol binding in hypothalamus and pituitary.
在卵巢切除且链脲佐菌素诱导糖尿病的大鼠中，外源性胰岛素撤除24小时会显著损害下丘脑-视前区和垂体全匀浆组分中雌二醇的摄取。胰岛素剥夺36小时后，相同组织的细胞核组分出现显著减少。随后恢复胰岛素治疗，在胰岛素替代24小时后，雌二醇摄取并未完全恢复。饮食中的脂肪含量对胰岛素剥夺36小时的糖尿病动物的脑或垂体雌二醇摄取没有影响。发现甘油三酯、酮体、葡萄糖、甘油和游离脂肪酸的循环水平在很大程度上可预测摄取水平，但没有单一代谢物能在所有组织中可靠地进行预测。这些数据表明，大鼠脑和垂体中雌二醇摄取的胰岛素依赖性变化发展缓慢，并且支持这样的假说，即糖尿病大鼠中观察到的至少一些生殖功能障碍可能是下丘脑和垂体中细胞核雌二醇结合受损的结果。
