Kuusi N
J Immunol Methods. 1979;31(3-4):361-4. doi: 10.1016/0022-1759(79)90149-2.
A simple antibody overlay technique for crossed immunoelectrophoresis is described. After the initial electrophoresis of antigens, the gel is overlaid with antibody-impregnated filter paper. This makes it necessary to pour only one gel, simplifying technique as compared with the conventional method.