Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy.
Department of Biosciences and Territory, University of Molise, 86090 Pesche, Italy.
Int J Mol Sci. 2024 Aug 6;25(16):8559. doi: 10.3390/ijms25168559.
As a nutraceutical, bovine lactoferrin (bLf), an iron-binding glycoprotein involved in innate immunity, is gaining elevated attention for its ability to exert pleiotropic functions and to be exceptionally tolerated even at high dosages. Some of bLf's activities, including its anti-inflammatory and antioxidant, are tightly linked to its ability to both chelate iron and enter inside the cell nucleus. Here, we present data about Valpalf, a new formulation containing bLf, sodium citrate, and sodium bicarbonate at a molar ratio of 10. In the present study, Valpalf exhibits superior iron-binding capacity, resistance to tryptic digestion, and a greater capacity to accumulate into the nucleus over time when compared to the native bLf alone. In agreement, Valpalf effectively reduces interleukin(IL)-6 levels in lipopolysaccharide-stimulated macrophages and modulates the expression of antioxidant enzymes, such as superoxide dismutase 1 and 2, in phorbol-12-myristate-13-acetate-stimulated monocytes. Of note, this potentiated bioactivity was corroborated in a retrospective study on the treatment of anemia of inflammation in hereditary thrombophilic pregnant and non-pregnant women, demonstrating that Valpalf improves hematological parameters and reduces serum IL-6 levels to a higher extent than bLf alone.
作为一种营养保健品，牛乳铁蛋白（bLf）是一种参与先天免疫的含铁糖蛋白，由于其具有发挥多种功能的能力，即使在高剂量下也能异常耐受，因此越来越受到关注。bLf 的一些活性，包括其抗炎和抗氧化作用，与其螯合铁和进入细胞核的能力密切相关。在这里，我们介绍了一种含有 bLf、柠檬酸钠和碳酸氢钠的新制剂 Valpalf，其摩尔比为 10。在本研究中，与单独的天然 bLf 相比，Valpalf 表现出更高的铁结合能力、抗胰蛋白酶消化能力和随着时间的推移积累到细胞核的更大能力。一致地，Valpalf 可有效降低脂多糖刺激的巨噬细胞中的白细胞介素（IL）-6 水平，并调节过氧化物酶 1 和 2 等抗氧化酶的表达，在佛波醇-12-肉豆蔻酸-13-乙酸刺激的单核细胞中。值得注意的是，在对遗传性血栓形成孕妇和非孕妇炎症性贫血的治疗进行的回顾性研究中证实了这种增强的生物活性，表明 Valpalf 改善了血液学参数，并比单独使用 bLf 更能降低血清 IL-6 水平。
