Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Stem Cell Biology and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
Immun Inflamm Dis. 2023 Aug;11(8):e972. doi: 10.1002/iid3.972.
Lactoferrin is a versatile protein with important modulatory functions in inflammation and immune response. This glycoprotein can bind and sequester iron and LPS, thereby intervening in certain signaling pathways and biological processes. In the present meta-analysis, we aimed to pool experimental data regarding the immunomodulatory effects of lactoferrin and its derived peptides on the NF-κB signaling pathway.
We searched PubMed, Google Scholar, and Web of Science databases and obtained all related articles published before April 2022. Finally, 25 eligible studies were selected, and their reports were analyzed.
We used Review Manager Version 5.2 to compute the standardized mean difference (SMD) and its 95% confidence interval. In addition, the source of heterogeneity was explored using meta-regression and sensitivity analysis. The symmetry of the funnel plot and Egger's test were also used to evaluate publication bias utilizing Comprehensive Meta-Analysis Version 2.
Comparing the group of cells and animals exposed to lipopolysaccharide alone with the group that received pretreatment with lactoferrin and its derivatives, we observed significant reductions in TNF-α, IL-1 beta, and IL-6 levels by 8.73 pg/mL, 2.21 pg/mL, and 3.24 pg/mL, respectively, in the second group. Additionally, IKK-β, p-IκB, and NF-κB (p65) levels were significantly lower by 7.37-fold, 15.02-fold, and 3.88-fold, respectively, in various cells and tissues.
Based on the results of this meta-analysis, lactoferrin and its derived peptides can be considered potent prophylactic and therapeutic candidates against inflammation-associated diseases by targeting the NF-kB pathway.
乳铁蛋白是一种多功能蛋白，在炎症和免疫反应中具有重要的调节功能。这种糖蛋白可以结合并隔离铁和 LPS，从而干预某些信号通路和生物过程。在本荟萃分析中，我们旨在汇总关于乳铁蛋白及其衍生肽对 NF-κB 信号通路的免疫调节作用的实验数据。
我们检索了 PubMed、Google Scholar 和 Web of Science 数据库，并获取了截至 2022 年 4 月之前发表的所有相关文章。最终，选择了 25 项符合条件的研究进行分析。
我们使用 Review Manager Version 5.2 计算标准化均数差（SMD）及其 95%置信区间。此外，还使用元回归和敏感性分析探索了异质性的来源。还使用综合荟萃分析版本 2 中的漏斗图对称性和 Egger 检验评估发表偏倚。
与单独接受脂多糖处理的细胞和动物组相比，接受乳铁蛋白及其衍生物预处理的组中 TNF-α、IL-1β 和 IL-6 水平分别降低了 8.73pg/mL、2.21pg/mL 和 3.24pg/mL。此外，在各种细胞和组织中，IKK-β、p-IκB 和 NF-κB（p65）水平分别降低了 7.37 倍、15.02 倍和 3.88 倍。
根据这项荟萃分析的结果，乳铁蛋白及其衍生肽可以通过靶向 NF-kB 通路被视为针对炎症相关疾病的有效预防和治疗候选物。
