Li Jinhua, Su Xiaoxing, Wang Yinlei, Yang Wei, Pan Yu, Su Chenggang, Zhang Xingguo
Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education; College of Horticulture and Landscape Architechture, Southwest University, No. 2 Tiansheng Road, Beibei, Chongqing, 400715, China.
Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Genes Genomics. 2018 Jan;40(1):1-15. doi: 10.1007/s13258-017-0604-x. Epub 2017 Aug 14.
BTB (broad-complex, tramtrack, and bric-a-brac) family proteins are characterized by the presence of a protein-protein interaction BTB domain. BTB proteins have diverse functions, including transcriptional regulation, protein degradation, chromatin remodeling, and cytoskeletal regulation. However, little is known about this gene family in tomato (Solanum lycopersicum), the most important model plant for crop species. In this study, 38 BTB genes were identified based on tomato whole-genome sequence. Phylogenetic analysis of BTB proteins in tomato revealed that SlBTB proteins could be divided into at least 4 subfamilies. The SlBTB proteins contains 1-3 BTB domains, and several other types of functional domains, including KCTD (Potassium channel tetramerization domain-containing), the MATH (meprin and TRAF homology), ANK (Ankyrin repeats), NPR1 (nonexpressor of pathogenesis-related proteins1), NPH3 (Nonphototropic Hypocotyl 3), TAZ zinc finger, C-terminal Kelch, Skp1 and Arm (Armadillo/beta-catenin-like repeat) domains are also found in some tomato BTB proteins. Moreover, their expression patterns in tissues/stages, in response to different abiotic stress treatments and hormones were also investigated. This study provides the first comprehensive analysis of BTB gene family in the tomato genome. The data will undoubtedly be useful for better understanding the potential functions of BTB genes, and their possible roles in mediating hormone cross-talk and abiotic stress in tomato as well as in some other relative species.
BTB（broad-complex、tramtrack和bric-a-brac）家族蛋白的特征是存在蛋白质-蛋白质相互作用的BTB结构域。BTB蛋白具有多种功能，包括转录调控、蛋白质降解、染色质重塑和细胞骨架调控。然而，对于番茄（Solanum lycopersicum）这个作物物种中最重要的模式植物来说，对这个基因家族却知之甚少。在本研究中，基于番茄全基因组序列鉴定出了38个BTB基因。对番茄中BTB蛋白的系统发育分析表明，SlBTB蛋白可至少分为4个亚家族。SlBTB蛋白含有1至3个BTB结构域，以及其他几种功能结构域，包括KCTD（含钾通道四聚化结构域）、MATH（meprin和TRAF同源结构域）、ANK（锚蛋白重复序列）、NPR1（病程相关蛋白1的非表达子）、NPH3（非向光性下胚轴3）、TAZ锌指结构、C端Kelch结构、Skp1和Arm（犰狳/β-连环蛋白样重复序列）结构域，在一些番茄BTB蛋白中也有发现。此外，还研究了它们在组织/发育阶段的表达模式，以及对不同非生物胁迫处理和激素的响应。本研究首次对番茄基因组中的BTB基因家族进行了全面分析。这些数据无疑将有助于更好地理解BTB基因的潜在功能，以及它们在介导番茄以及其他一些相关物种中的激素互作和非生物胁迫方面可能发挥的作用。
