BTB-TAZ 蛋白 MdBT2 与 DELLA 蛋白 MdRGL3a 的相互作用调控硝酸盐介导的植物生长。
Interaction of BTB-TAZ protein MdBT2 and DELLA protein MdRGL3a regulates nitrate-mediated plant growth.
机构信息
State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong, 271018, China.
Qingdao Agricultural University, Qingdao, Shandong, 266109, China.
出版信息
Plant Physiol. 2021 May 27;186(1):750-766. doi: 10.1093/plphys/kiab065.
Nitrate acts as a vital signal molecule in the modulation of plant growth and development. The phytohormones gibberellin (GA) is also involved in this process. However, the exact molecular mechanism of how nitrate and GA signaling pathway work together in regulating plant growth remains poorly understood. In this study, we found that a nitrate-responsive BTB/TAZ protein MdBT2 participates in regulating nitrate-induced plant growth in apple (Malus × domestica). Yeast two-hybridization, protein pull-down, and bimolecular fluorescence complementation (BiFC) assays showed that MdBT2 interacts with a DELLA protein MdRGL3a, which is required for the ubiquitination and degradation of MdRGL3a proteins via a 26S proteasome-dependent pathway. Furthermore, heterologous expression of MdBT2 partially rescued growth inhibition caused by overexpression of MdRGL3a in Arabidopsis. Taken together, our findings indicate that MdBT2 promotes nitrate-induced plant growth partially through reducing the abundance of the DELLA protein MdRGL3a.
硝酸盐作为一种重要的信号分子，参与植物生长和发育的调节。植物激素赤霉素（GA）也参与这一过程。然而，硝酸盐和 GA 信号通路如何协同调节植物生长的具体分子机制仍知之甚少。在这项研究中，我们发现一种对硝酸盐有反应的 BTB/TAZ 蛋白 MdBT2 参与调节苹果（Malus × domestica）中硝酸盐诱导的生长。酵母双杂交、蛋白 pull-down 和双分子荧光互补（BiFC）实验表明，MdBT2 与一个 DELLA 蛋白 MdRGL3a 相互作用，MdRGL3a 蛋白通过 26S 蛋白酶体依赖的途径进行泛素化和降解。此外，MdBT2 的异源表达部分挽救了过量表达 MdRGL3a 引起的拟南芥生长抑制。综上所述，我们的研究结果表明，MdBT2 通过降低 DELLA 蛋白 MdRGL3a 的丰度来促进硝酸盐诱导的植物生长。
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