College of Horticulture, Hebei Agricultural University, Baoding 071001, China.
Research Center for Pear Engineering and Technology of Hebei Province, Baoding 071001, China.
Genes (Basel). 2024 Jul 23;15(8):968. doi: 10.3390/genes15080968.
Protein disulfide isomerases (PDIs) and PDI-like proteins catalyze the oxidation and reduction in protein disulfide bonds, inhibit aggregation of misfolded proteins, and participate in isomerization and abiotic stress responses. The wild type 'duli' pear () is an important rootstock commonly used for commercial pear tree grafting in northern China. In this study, we identified 24 genes, named , from the genome of 'duli' pear. With 12 homologous gene pairs, these 24 distribute on 12 of its 17 chromosomes. Phylogenetic analysis placed the 24 PbPDIs into four clades and eleven groups. Collinearity analysis of the between , , and revealed that the of 'duli' pear show a strong collinear relationship with those from Arabidopsis, a dicot; but a weak collinear relationship with those from rice, a monocot. Quantitative RT-PCR analysis showed that most of the were upregulated by salt stress. Identification and expression analysis of 'duli' pear under salt stress conditions could provide useful information for further research in order to generate salt-resistant rootstock for pear grafting in the future.
蛋白质二硫键异构酶（PDI）和 PDI 样蛋白催化蛋白质中二硫键的氧化和还原，抑制错误折叠蛋白质的聚集，并参与异构化和非生物胁迫反应。野生型‘独丽’梨（）是中国北方商业梨树嫁接的重要砧木。本研究从‘独丽’梨基因组中鉴定出 24 个基因，命名为。与 12 对同源基因相比，这 24 个基因分布在其 17 条染色体中的 12 条上。系统发育分析将 24 个 PbPDIs 分为四个分支和十一个组。对、、和之间的共线性分析表明，‘独丽’梨的显示出与拟南芥（双子叶植物）的强共线性关系，但与水稻（单子叶植物）的弱共线性关系。定量 RT-PCR 分析表明，大多数在盐胁迫下上调。在盐胁迫条件下‘独丽’梨的鉴定和表达分析可为未来生成用于梨树嫁接的耐盐砧木的研究提供有用信息。
