College of Horticulture, Hebei Agricultural University, Baoding 071001, China.
Int J Mol Sci. 2024 Jul 21;25(14):7967. doi: 10.3390/ijms25147967.
'Duli' ( Bunge) is one of the main rootstocks of pear trees in China. Gibberellin (GA) is a key plant hormone and the roles of GA in nitrate (NO) uptake and metabolism in plants remain unclear. In this study, we investigated the effects of exogenous GA on the N metabolism of 'Duli' seedlings under NO deficiency. The results showed that exogenous GA significantly improves 'Duli' growth under NO deficiency. On the one hand, GA altered the root architecture, increased the content of endogenous hormones (GA, IAA, and ZR), and enhanced photosynthesis; on the other hand, it enhanced the activities of N-metabolizing enzymes and the accumulation of N, and increased the expression levels of N absorption () and the metabolism genes (, , , and ). However, GA did not delay the degradation of chlorophyll. Paclobutrazol had the opposite effect on growth. Overall, GA can increase NO uptake and metabolism and relieve the growth inhibition of 'Duli' seedlings under NO deficiency.
“杜梨”(Bunge)是中国梨树的主要砧木之一。赤霉素(GA)是一种关键的植物激素,但 GA 在植物硝酸盐(NO)吸收和代谢中的作用尚不清楚。本研究探讨了外源 GA 对 NO 缺乏下‘杜梨’幼苗氮代谢的影响。结果表明,外源 GA 可显著改善 NO 缺乏下‘杜梨’的生长。一方面,GA 改变了根系结构,增加了内源激素(GA、IAA 和 ZR)的含量,增强了光合作用;另一方面,GA 增强了氮代谢酶的活性和氮的积累,增加了氮吸收相关基因(、、和)和氮代谢相关基因(、、和)的表达水平。然而,GA 并没有延缓叶绿素的降解。多效唑则对生长有相反的影响。总体而言,GA 可增加 NO 的吸收和代谢,缓解 NO 缺乏下‘杜梨’幼苗的生长抑制。