基因组 IEGM1362 的生物信息学分析，一种（-）-异胡薄荷醇生物转化器。

Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, 13 Golev Str., 614081 Perm, Russia.

Department of Microbiology and Immunology, Perm State University, 15 Bukirev Str., 614990 Perm, Russia.

Genes (Basel). 2024 Jul 28;15(8):992. doi: 10.3390/genes15080992.

A genome of IEGM 1362 was sequenced and annotated. This strain can transform monoterpene alcohol (-)-isopulegol with the formation of two novel pharmacologically promising metabolites. Nine genes encoding cytochrome P450, presumably involved in (-)-isopulegol transformation, were found in the genome of IEGM 1362. Primers and PCR conditions for their detection were selected. The obtained data can be used for the further investigation of genes encoding enzymes involved in monoterpene biotransformation.

对 IEGM 1362 的基因组进行了测序和注释。该菌株可以转化单萜醇(-)-异胡薄荷醇，形成两种具有潜在药用价值的新型代谢产物。在 IEGM 1362 的基因组中发现了 9 个编码细胞色素 P450 的基因，这些基因可能参与了(-)-异胡薄荷醇的转化。选择了用于检测它们的引物和 PCR 条件。获得的数据可用于进一步研究参与单萜生物转化的酶编码基因。