Nagy I, Compernolle F, Ghys K, Vanderleyden J, De Mot R
Department of Applied Plant Sciences, Catholic University of Leuven, Heverlee, Belgium.
Appl Environ Microbiol. 1995 May;61(5):2056-60. doi: 10.1128/aem.61.5.2056-2060.1995.
During atrazine degradation by Rhodococcus sp. strain N186/21, N-dealkylated metabolites and an hydroxyisopropyl derivative are produced. The cytochrome P-450 system that is involved in degradation of thiocarbamate herbicides by strain N186/21 (I. Nagy, G. Schoofs, F. Compernolle, P. Proost, J. Vanderleyden, and R. De Mot, J. Bacteriol. 177:676-687, 1995) is also required for atrazine degradation. Atrazine-degrading activity was conferred on the atrazine-negative strains, mutant FAJ2027 of Rhodococcus sp. strain N186/21 and Rhodococcus erythropolis SQ1, upon transformation with the genes encoding the cytochrome P-450 system.
在红球菌属菌株N186/21降解莠去津的过程中,会产生N-脱烷基代谢物和一种羟基异丙基衍生物。红球菌属菌株N186/21降解硫代氨基甲酸盐类除草剂时所涉及的细胞色素P-450系统(I. 纳吉、G. 舒夫斯、F. 孔佩诺勒、P. 普罗斯特、J. 万德雷登和R. 德莫特,《细菌学杂志》177:676 - 687,1995年)对于莠去津的降解也是必需的。在用编码细胞色素P-450系统的基因转化后,莠去津降解活性赋予了莠去津阴性菌株,即红球菌属菌株N186/21的突变体FAJ2027和红平红球菌SQ1。