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灵芝三萜通过调控 CREB 和 MAPK 信号通路对 B16F10 细胞的黑色素生成抑制作用

The Anti-Melanogenic Effects of Ganodermanontriol from the Medicinal Mushroom through the Regulation of the CREB and MAPK Signaling Pathways in B16F10 Cells.

机构信息

Department of Medicinal Biosciences, Research Institute for Biomedical & Health Science, College of Biomedical and Health Science, Konkuk University, 268 Chungwon-daero, Chungju-si 27478, Republic of Korea.

Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, 92, Bisan-ro, Eumseong-gun 27709, Republic of Korea.

出版信息

Molecules. 2024 Aug 22;29(16):3976. doi: 10.3390/molecules29163976.

DOI:10.3390/molecules29163976
PMID:39203053
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11357533/
Abstract

, a member of the Basidiomycetes family, is attracting attention for its medicinal potential due to its biological activity and the presence of numerous bioactive compounds. Although it is known that extracts of this mushroom inhibit melanin production, there are few reports on a single substance associated with this effect. In this study, we identified ganodermanontriol (GT), a novel compound from , that effectively inhibited melanin biosynthesis in B16F10 cells. GT inhibits melanin production by suppressing the expression of cellular tyrosinase proteins and microphthalmia-related transcription factor (MITF). Furthermore, GT affects the phosphorylation of cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB) and mitogen-activated protein kinase (MAPK) signaling molecules, which are involved in melanogenesis in B16F10 cells. Finally, the biosynthesis of GT and other substances by was evaluated using HPLC analysis. Thus, this study revealed the mechanism by which GT in inhibits melanin production in B16F10 cells, and these findings will contribute to promoting the potential use of this mushroom in the future.

摘要

灵芝，属于担子菌纲真菌，由于其生物活性和含有多种生物活性化合物而引起了人们对其药用潜力的关注。虽然已知该蘑菇的提取物能抑制黑色素的生成，但与这种作用相关的单一物质的报道很少。在这项研究中，我们从灵芝中鉴定出了灵芝三醇（GT），这是一种新型化合物，能有效抑制 B16F10 细胞中的黑色素生物合成。GT 通过抑制细胞酪氨酸酶蛋白和小眼畸形相关转录因子（MITF）的表达来抑制黑色素的产生。此外，GT 还影响参与 B16F10 细胞中黑色素生成的环磷酸腺苷（cAMP）反应元件结合蛋白（CREB）和丝裂原活化蛋白激酶（MAPK）信号分子的磷酸化。最后，采用 HPLC 分析评估了灵芝中 GT 和其他物质的生物合成。因此，本研究揭示了 GT 在灵芝中抑制 B16F10 细胞中黑色素生成的作用机制，这些发现将有助于促进该蘑菇在未来的潜在应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00e8/11357533/b6016ac06c1c/molecules-29-03976-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00e8/11357533/0c19b346fc68/molecules-29-03976-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00e8/11357533/18f1a3b664c8/molecules-29-03976-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00e8/11357533/a4dcf825a6fd/molecules-29-03976-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00e8/11357533/0ae892b92a9c/molecules-29-03976-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00e8/11357533/a9a15ac30cc1/molecules-29-03976-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00e8/11357533/789a8d58fc16/molecules-29-03976-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00e8/11357533/b6016ac06c1c/molecules-29-03976-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00e8/11357533/0c19b346fc68/molecules-29-03976-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00e8/11357533/18f1a3b664c8/molecules-29-03976-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00e8/11357533/a4dcf825a6fd/molecules-29-03976-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00e8/11357533/0ae892b92a9c/molecules-29-03976-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00e8/11357533/a9a15ac30cc1/molecules-29-03976-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00e8/11357533/789a8d58fc16/molecules-29-03976-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00e8/11357533/b6016ac06c1c/molecules-29-03976-g007.jpg

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本文引用的文献

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A Review of Triterpenoids and Their Bioactivities.三萜类化合物及其生物活性研究进展
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