Kishi H, Inui S, Muraguchi A, Hirano T, Yamamura Y, Kishimoto T
J Immunol. 1985 May;134(5):3104-7.
A human B cell clone (SGB3) responsive to IL 2 was established. Both recombinant IL 2 and B cell differentiation factor (BCDF) induced IgG secretion in SGB3 cells in a dose-dependent manner, but IL 2 did not affect the proliferation of SGB3 cells. FACS analysis showed that SGB3 cells expressed Tac antigen and anti-Tac antibody inhibited IL 2-induced IgG secretion without any inhibitory effect on BCDF-induced IgG secretion. These results showed that IL 2 could directly act on B cells and provide a differentiation signal through IL 2 receptors distinct from BCDF receptors. Physiologic relevance of IL 2 in the antibody response was discussed.
建立了一株对白细胞介素2（IL-2）有反应的人B细胞克隆（SGB3）。重组IL-2和B细胞分化因子（BCDF）均以剂量依赖方式诱导SGB3细胞分泌IgG，但IL-2不影响SGB3细胞的增殖。荧光激活细胞分选（FACS）分析显示，SGB3细胞表达Tac抗原，抗Tac抗体抑制IL-2诱导的IgG分泌，而对BCDF诱导的IgG分泌无任何抑制作用。这些结果表明，IL-2可直接作用于B细胞，并通过与BCDF受体不同的IL-2受体提供分化信号。文中讨论了IL-2在抗体应答中的生理相关性。
