Tanaka T, Saiki O, Doi S, Hatakeyama M, Doi T, Kono T, Mori H, Fujii M, Sugamura K, Negoro S
Third Department of Internal Medicine, Osaka University, Japan.
Eur J Immunol. 1987 Sep;17(9):1379-82. doi: 10.1002/eji.1830170925.
Human B lymphoblastoid line, SKW 6-4, cells were induced to IgM-secreting cells by high concentrations of interleukin 2 (IL 2). These cells were found to be unreactive with anti-Tac antibody and did not express mRNA detectable for Tac antigen. In Scatchard plot analysis, low-affinity IL 2-binding sites were found on SKW 6-4 cells. Moreover, analysis of the IL 2-binding molecules revealed ones (molecular weight 70,000 and 75,000) distinct from Tac antigen. It is conceivable that IL 2 exerts its effect through its interaction with these novel IL 2-binding molecules in SKW 6-4 cells.
