Li Shinuo, Wang Qingfeng, Mi Jinqiu, Chen Haotian, Yuan Tianhao, Wang Yue, Zhao Lihong, Ma Qiugang, Huang Shimeng
National Key Laboratory of Livestock and Poultry Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Laboratory of Feedgrain Safety and Healthy Poultry Farming, Beijing Jingwa Agricultural Science and Technology Innovation Center, Beijing 101206, China.
Microorganisms. 2024 Jul 30;12(8):1559. doi: 10.3390/microorganisms12081559.
Oviductal inflammation (OI) significantly reduces the egg production and economic returns in poultry farming. While (LAC) is effective against inflammation, its role in treating or preventing oviductal inflammation is understudied. In this study, we investigated the therapeutic mechanisms of LAC on oviductal inflammation, with a focus on reproductive tract health, microbiome, gene expression, and cytokine levels. This study involved 24 Jingfen No. 6 laying hens aged 60 weeks, divided into four groups: the CON, OI, OI + LAC, and OI + heat-killed (HLAC) groups. And it included a 10-day adaptation, a 7-day period for the development of OI using inflammation-inducing drugs (the control received saline), followed by an 8-day treatment in which the CON and OI groups received 1 mL of MRS broth daily, and the OI + LAC and OI + HLAC groups were treated with live and heat-killed (10 CFUs/mL), respectively, with six hens in each group. This study showed that supplementation significantly reduced the oviductal inflammation and atrophy in the hens, with the affected hens showing markedly lower egg production rates ( < 0.001) compared to the control and treated groups (OI + HLAC and OI + LAC). The daily intake of fresh (OI + LAC, = 0.076) or heat-killed (OI + HLAC, < 0.01) notably enhanced the feed conversion efficiency. The OI group suffered significant ovarian damage and vascular rupture, more so than the CON group, while supplementation mitigated this damage. The IL-1β, IL-6, and IL-8 levels were significantly elevated in the OI group compared to those in the OI + LAC group ( < 0.05), with a significant reduction in the TNF-α levels in the latter ( < 0.001). The supplementation improved the microbial composition in the cecum, isthmus, and shell gland, enriching the cecum with beneficial bacteria, such as and . This approach fostered ovarian health and follicle differentiation and preserved the epithelial cell barrier function in the shell gland, reducing inflammatory damage in the genital tract. This dual efficacy underscores the role of the probiotic in diminishing oviductal inflammation, regardless of its state.
输卵管炎（OI）显著降低了家禽养殖中的产蛋量和经济效益。虽然嗜酸乳杆菌（LAC）对炎症有效，但其在治疗或预防输卵管炎方面的作用尚未得到充分研究。在本研究中，我们调查了LAC对输卵管炎的治疗机制，重点关注生殖道健康、微生物群、基因表达和细胞因子水平。本研究涉及24只60周龄的京粉6号蛋鸡，分为四组：对照组（CON）、OI组、OI + LAC组和OI + 热灭活嗜酸乳杆菌（HLAC）组。实验包括10天的适应期，使用炎症诱导药物诱导OI 7天（对照组接受生理盐水），随后进行8天的治疗，CON组和OI组每天接受1 mL MRS肉汤，OI + LAC组和OI + HLAC组分别用活的和热灭活的嗜酸乳杆菌（10⁸CFUs/mL）治疗，每组6只母鸡。本研究表明，补充嗜酸乳杆菌显著降低了母鸡的输卵管炎症和萎缩，与对照组和治疗组（OI + HLAC和OI + LAC）相比，受影响的母鸡产蛋率明显较低（P < 0.001）。每天摄入新鲜的（OI + LAC，P = 0.076）或热灭活的（OI + HLAC，P < 0.01）嗜酸乳杆菌显著提高了饲料转化效率。OI组遭受了比CON组更严重的卵巢损伤和血管破裂，而补充嗜酸乳杆菌减轻了这种损伤。与OI + LAC组相比，OI组的IL-1β、IL-6和IL-8水平显著升高（P < 0.05），而后者的TNF-α水平显著降低（P < 0.001）。补充嗜酸乳杆菌改善了盲肠、峡部和壳腺的微生物组成，使盲肠中有益细菌如双歧杆菌属和乳杆菌属富集。这种方法促进了卵巢健康和卵泡分化，并维持了壳腺中的上皮细胞屏障功能，减少了生殖道中的炎症损伤。这种双重功效突出了益生菌在减轻输卵管炎方面的作用，无论其状态如何。
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