Pomari Elena, Orza Pierantonio, Bernardi Milena, Fracchetti Fabio, Campedelli Ilenia, De Marta Patrick, Recchia Alessandra, Paradies Paola, Buonfrate Dora
Department of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.
Microbion srl, San Giovanni Lupatoto, 37057 Verona, Italy.
Microorganisms. 2024 Aug 6;12(8):1603. doi: 10.3390/microorganisms12081603.
Differentiating between species is relevant in human medicine. toxins might be effective against , a nematode causing relevant human morbidity. Our first objective was to evaluate genomic and MALDI-TOF identification methods for . Our secondary objective was to evaluate a possible negative selection pressure of against . PCR and Sanger were compared to MALDI-TOF on a collection of 44 group strains. toxin genes were searched on 17 stool samples from -infected and uninfected dogs. Metagenomic rRNA was used for microbiome composition. The inter-rate agreement between PCR, Sanger, and MALDI-TOF was 0.631 k (-value = 6.4 × 10). toxins were not found in dogs' stool. and were the major phyla in the dogs' microbiome (both represented >20% of the total bacterial community). was underrepresented in all -positive dogs. However, the general composition of bacterial communities was not significantly linked with infection. The genomic methods allowed accurate differentiation between and . There was no association between and infection, but further studies are needed to confirm this finding. We provide the first descriptive results about bacterial fecal composition in dogs with infection.
区分物种在人类医学中具有重要意义。毒素可能对某种导致人类相关发病的线虫有效。我们的首要目标是评估用于该线虫的基因组和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）鉴定方法。我们的次要目标是评估该线虫对某种物质可能存在的负选择压力。在一组44株该线虫菌株上，将聚合酶链反应（PCR）和桑格测序法与MALDI-TOF进行了比较。在17份来自感染和未感染该线虫的犬只的粪便样本中搜索毒素基因。宏基因组核糖体RNA用于分析微生物群落组成。PCR、桑格测序法和MALDI-TOF之间的组内一致性为0.631 k（P值 = 6.4 × 10）。在犬只粪便中未发现毒素。拟杆菌门和厚壁菌门是犬只微生物群落中的主要门类（两者均占细菌群落总数的>20%）。在所有感染该线虫呈阳性的犬只中，某一菌门的占比过低。然而，细菌群落的总体组成与该线虫感染并无显著关联。基因组方法能够准确区分该线虫的不同种类。该线虫感染与某一因素之间没有关联，但需要进一步研究来证实这一发现。我们提供了关于感染该线虫的犬只粪便细菌组成的首批描述性结果。
