Escuela de Medicina Veterinaria, Facultad de Ciencias de la Vida, Universidad Nacional Andrés Bello., Santiago, Chile.
Chemical and Bioprocess Engineering, Pontificia Universidad Católica de Chile, Santiago, Chile.
PeerJ. 2022 Feb 16;10:e12695. doi: 10.7717/peerj.12695. eCollection 2022.
Obesity is a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries. It is estimated that over 40% of the canine population suffers from obesity, which manifests in an increased risk of chronic osteoarticular, metabolic, and cardiovascular diseases. The intestinal microbiome of obese animals shows increases in the abundance of certain members capable of extracting energy from complex polysaccharides. The objective of this study was to compare the composition and predicted function of the intestinal microbiome of Chilean obese and normal weight adult dogs. Twenty clinically healthy dogs were classified according to their body condition score (BCS) as obese ( = 10) or normal weight ( = 10). DNA was extracted from stool samples, followed by next-generation sequencing of the 16S rRNA V3-V4 region and bioinformatics analysis targeting microbiome composition and function. Significant differences were observed between these groups at the phylum level, with anincrease in Firmicutes and a decrease in Bacteroidetes in obese dogs. Microbiome compositions of these animals correlated with their BCS, and obese dogs showed enrichment in pathways related to transport, chemotaxis, and flagellar assembly. These results highlight the differences in the gut microbiome between normal weight and obese dogs and prompt further research to improve animal health by modulating the gut microbiome.
肥胖症是一种多因素营养障碍，在狗中高发，在发达国家和发展中国家都有观察到。据估计，超过 40%的犬群患有肥胖症，这会增加患慢性骨关节炎、代谢和心血管疾病的风险。肥胖动物的肠道微生物组中，某些能够从复杂多糖中提取能量的成员的丰度增加。本研究的目的是比较智利肥胖和正常体重成年犬的肠道微生物组的组成和预测功能。根据身体状况评分（BCS），将 20 只临床健康的狗分为肥胖（=10）或正常体重（=10）。从粪便样本中提取 DNA，然后对 16S rRNA V3-V4 区域进行下一代测序，并针对微生物组组成和功能进行生物信息学分析。在门水平上观察到这些组之间存在显著差异，肥胖犬的厚壁菌门增加，拟杆菌门减少。这些动物的微生物组组成与其 BCS 相关，肥胖犬在与运输、趋化和鞭毛组装相关的途径中富集。这些结果突出了正常体重和肥胖犬之间肠道微生物组的差异，并促使进一步研究通过调节肠道微生物组来改善动物健康。
