Olivas-Flores Jocelyn, Chávez-Méndez José Román, Castillo-Martínez Nydia Alejandra, Sánchez-Pérez Héctor Javier, Serrano-Medina Aracely, Cornejo-Bravo José Manuel
Faculty of Chemical Sciences and Engineering, Autonomous University of Baja California, Calzada Universidad 14418, Parque Industrial Internacional, Tijuana 22424, Mexico.
Faculty of Health Sciences, Autonomous University of Baja California, Blvd Universitario No. 1000, Valle San Pedro, Tijuana 21500, Mexico.
Microorganisms. 2024 Aug 6;12(8):1605. doi: 10.3390/microorganisms12081605.
This study evaluates the antimicrobial efficacy of chitosan nanoparticles (CNPs), varying in size, against clinical isolates of (MTB), , , , and , as well as the antimicrobial effects of aqueous extracts and lyophilized powders of (garlic) species. CNPs were synthesized through ionotropic gelation and characterized by Z potential, hydrodynamic diameter (dynamic light scattering, DLS), and SEM. Aqueous garlic extracts were prepared via decoction. We assessed antimicrobial activity using disk diffusion and broth microdilution methods; in addition, a modified agar proportion method in blood agar was used for antimicrobial activity against MTB. CNPs inhibited MTB growth at 300 μg for 116.6 nm particles and 400 μg for 364.4 nm particles. The highest antimicrobial activity was observed against with nanoparticles between 200 and 280 nm. extract produced inhibition for at 100 μg. The results indicate that CNPs possess significant antimicrobial properties against a range of pathogens, including MTB, at high concentrations. On the other hand, aqueous extracts exhibited antimicrobial activity. Nonetheless, due to their instability in solution, the use of lyophilized powder is preferable.
本研究评估了不同尺寸的壳聚糖纳米颗粒（CNPs）对结核分枝杆菌（MTB）、金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和白色念珠菌临床分离株的抗菌效果，以及大蒜品种水提取物和冻干粉末的抗菌作用。通过离子凝胶法合成CNPs，并通过Z电位、流体动力学直径（动态光散射，DLS）和扫描电子显微镜（SEM）对其进行表征。通过煎煮法制备大蒜水提取物。我们使用纸片扩散法和肉汤微量稀释法评估抗菌活性；此外，在血琼脂中使用改良琼脂比例法评估对MTB的抗菌活性。对于粒径为116.6 nm的颗粒，300 μg的CNPs可抑制MTB生长；对于粒径为364.4 nm的颗粒，400 μg可抑制其生长。观察到粒径在200至280 nm之间的纳米颗粒对金黄色葡萄球菌具有最高的抗菌活性。大蒜提取物在100 μg时对大肠杆菌产生抑制作用。结果表明，高浓度的CNPs对包括MTB在内的一系列病原体具有显著的抗菌性能。另一方面，大蒜水提取物表现出抗菌活性。然而，由于其在溶液中的不稳定性，使用大蒜冻干粉末更为可取。
