Das Bhabani Shankar, Sarangi Ashirbad, Panigrahi Lipsa Leena, Nayak Sunita, Arakha Manoranjan, Bhattacharya Debapriya
Center for Biotechnology, Siksha 'O' Anusandhan (Deemed to be University), Bhubaneswar, 751003, Odisha, India.
Institute of Life Sciences, Bhubaneswar, 751021, Odisha, India.
Curr Top Med Chem. 2025;25(9):1105-1119. doi: 10.2174/0115680266348684241211072446.
INTRODUCTION/OBJECTIVE: Tuberculosis (TB) remains a persistent global health challenge, with an increasing incidence of cases and limitations in current treatment strategies. Traditional therapy involves long drug treatments that can cause side effects and lead to drug-resistant strains, making treatment less effective. This study aimed to explore the therapeutic potential of a novel nanoparticle-based delivery system for Thymol (THY), a natural antibacterial bioactive molecule, to combat , a model organism for .
A nanoparticle-based delivery system was developed using biocompatible Thymolconjugated Chitosan Zinc Ferrite Nanoparticles (THY-CH-ZnFeO NPs). The nanoconjugates were characterized for their morphological and chemical properties.
The characterization of synthesised nanoparticles showed THY-CH-ZnFeO NPs to exhibit enhanced biocompatibility and antibacterial activity against compared to THY alone. The nanoconjugates induced Reactive Oxygen Species (ROS)-mediated damage to the bacterial cell membrane, effectively inhibiting bacterial replication, dormancy, and biofilm formation. Additionally, the nanoconjugates demonstrated low cytotoxicity towards the human kidney cell line.
The study's findings highlighted a new direction for developing nanoparticle-based antimycobacterial agents with a wide application in treating TB and other bacterial diseases. The THY-CH-ZnFe2O4 NPs show promise as a safe and effective therapeutic agent, offering a potential solution to the limitations of current TB treatment strategies.
引言/目的：结核病仍然是一项持续存在的全球卫生挑战，病例发病率不断上升，且当前治疗策略存在局限性。传统疗法需要长期药物治疗，这可能会导致副作用并产生耐药菌株，使治疗效果降低。本研究旨在探索一种基于新型纳米颗粒的递送系统对百里酚（THY）的治疗潜力，百里酚是一种天然抗菌生物活性分子，以对抗[未提及具体对抗对象]，作为[未提及具体模型生物]的模型生物。
使用生物相容性的百里酚共轭壳聚糖锌铁氧体纳米颗粒（THY-CH-ZnFeO NPs）开发了一种基于纳米颗粒的递送系统。对纳米缀合物的形态和化学性质进行了表征。
合成纳米颗粒的表征显示，与单独的百里酚相比，THY-CH-ZnFeO NPs表现出增强的生物相容性和对[未提及具体细菌]的抗菌活性。纳米缀合物诱导活性氧（ROS）介导的细菌细胞膜损伤，有效抑制细菌复制、休眠和生物膜形成。此外，纳米缀合物对人肾细胞系表现出低细胞毒性。
该研究结果突出了开发基于纳米颗粒的抗分枝杆菌药物的新方向，在治疗结核病和其他细菌疾病方面具有广泛应用。THY-CH-ZnFe2O4 NPs有望成为一种安全有效的治疗剂，为当前结核病治疗策略的局限性提供潜在解决方案。
