Onyeaghala Guillaume C, Sharma Shweta, Oyenuga Mosunmoluwa, Staley Christopher M, Milne Ginger L, Demmer Ryan T, Shaukat Aasma, Thyagarajan Bharat, Straka Robert J, Church Timothy R, Prizment Anna E
Division of Nephrology, Hennepin Healthcare, University of Minnesota, Minneapolis, MN 55415, USA.
Division of Epidemiology and Community Health, University of Minnesota, Minneapolis, MN 55455, USA.
Microorganisms. 2024 Aug 7;12(8):1609. doi: 10.3390/microorganisms12081609.
Several bacterial taxa enriched in inflammatory bowel diseases and colorectal cancer (CRC) are found in the oral cavity. We conducted a pilot study nested within a six-week aspirin intervention in a randomized placebo-controlled trial to test their response to aspirin intervention. Fifty healthy subjects, 50-75 years old, were randomized to receive 325 mg aspirin (n = 30) or placebo (n = 20) orally once daily for six weeks. Oral tongue swabs were collected at baseline and week six. We estimated the association between aspirin use and the temporal changes in the relative abundance of pre-specified genus level taxa from pre- to post-treatment. The temporal change in relative abundance differed for eight genus level taxa between the aspirin and placebo groups. In the aspirin group, there were significant increases in the relative abundances of , , , and and significant decreases in , , , and relative to placebo. The log ratio of to declined more in the aspirin group than placebo, signaling a potential marker associated with aspirin intervention. These preliminary findings should be validated using metagenomic sequencing and may guide future studies on the role of aspirin on taxa in various oral ecological niches.
在口腔中发现了几种在炎症性肠病和结直肠癌（CRC）中富集的细菌分类群。我们在一项随机安慰剂对照试验的为期六周的阿司匹林干预中进行了一项嵌套式试点研究，以测试它们对阿司匹林干预的反应。五十名50至75岁的健康受试者被随机分为两组，一组每天口服一次325毫克阿司匹林（n = 30），另一组口服安慰剂（n = 20），持续六周。在基线和第六周采集口腔舌拭子。我们估计了阿司匹林使用与治疗前至治疗后预先指定的属水平分类群相对丰度的时间变化之间的关联。阿司匹林组和安慰剂组之间，八个属水平分类群的相对丰度时间变化有所不同。在阿司匹林组中，相对于安慰剂，[具体分类群1]、[具体分类群2]、[具体分类群3]和[具体分类群4]的相对丰度显著增加，而[具体分类群5]、[具体分类群6]、[具体分类群7]和[具体分类群8]的相对丰度显著降低。阿司匹林组中[具体分类群9]与[具体分类群10]的对数比下降幅度大于安慰剂组，这表明其可能是与阿司匹林干预相关的一个潜在标志物。这些初步发现应通过宏基因组测序进行验证，并可能为未来关于阿司匹林在各种口腔生态位中对分类群作用的研究提供指导。
