Yang Ke, Wang Yuezhu, Zhang Shizhou, Zhang Dongsheng, Hu Lihua, Zhao Tengda, Zheng Huajun
Department of Oral and Maxillofacial Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
Department of Health Management Center, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
Front Microbiol. 2021 Oct 12;12:719601. doi: 10.3389/fmicb.2021.719601. eCollection 2021.
Oral microbiota dysbiosis is associated with the occurrence and progression of oral cancer. To investigate the association between the microbiota and risk of oral squamous cell carcinoma (OSCC), we identified the microbial composition of paired tumor (TT)/normal paracancerous tissues (NPT) and saliva (TS) samples in OSCC patients through 16S rRNA gene sequencing. A total of 22 phyla, 321 genera, and 869 species were identified in the oral samples. Paired comparisons revealed significant differences between TT, NPT, and TS groups, with the genus significantly enriched in TT. The phylum Actinobacteria; genus ; and species , , and were significantly enriched in NPT, while the phylum Bacteroidetes; genera , , and ; and seven species, including sp., sp., and sp., were significantly enriched in TS. In TTs, the abundance of was profoundly higher in the gingiva, while and were enriched in the lining mucosa and tongue. Increasing in abundance from the early tumor stage to the late stage, in TT and sp. in TS were positively correlated with OSCC development, suggesting that bacteria were selected by different microenvironments. The correlation between 11 microbial species and 17 pathway abundances was revealed, indicating the potential function of low-abundance bacteria. Overall, our analysis revealed that multiple oral bacterial taxa are associated with a subsequent risk of OSCC and may be used as biomarkers for risk prediction and intervention in oral cancers.
口腔微生物群失调与口腔癌的发生和发展相关。为了研究微生物群与口腔鳞状细胞癌（OSCC）风险之间的关联，我们通过16S rRNA基因测序确定了OSCC患者配对的肿瘤组织（TT）/正常癌旁组织（NPT）和唾液（TS）样本的微生物组成。在口腔样本中总共鉴定出22个门、321个属和869个物种。配对比较显示TT、NPT和TS组之间存在显著差异，某属在TT中显著富集。放线菌门；某属；以及某些物种在NPT中显著富集，而拟杆菌门；某些属；以及七个物种，包括某菌属、某菌属和某菌属，在TS中显著富集。在TT中，某菌在牙龈中的丰度显著更高，而某菌和某菌在衬里黏膜和舌头中富集。从肿瘤早期到晚期丰度增加，TT中的某菌和TS中的某菌属与OSCC发展呈正相关，表明细菌是由不同的微环境选择的。揭示了11种微生物物种与17种通路丰度之间的相关性，表明低丰度细菌的潜在功能。总体而言，我们的分析表明多种口腔细菌类群与随后的OSCC风险相关，并且可用作口腔癌风险预测和干预的生物标志物。
